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A. Hoekema, P. Hirsch, P. Hooykaas, R. Schilperoort (1983)
A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 303
A. Walmsley, M. Alvarez, Y. Jin, D. Kirk, S. Lee, J. Pinkhasov, M. Rigano, C. Arntzen, H. Mason (2003)
Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomatoPlant Cell Reports, 21
M. Horwitz, G. Harth, B. Dillon, Saša Masleša-Galić (2000)
Recombinant bacillus calmette-guerin (BCG) vaccines expressing the Mycobacterium tuberculosis 30-kDa major secretory protein induce greater protective immunity against tuberculosis than conventional BCG vaccines in a highly susceptible animal model.Proceedings of the National Academy of Sciences of the United States of America, 97 25
A. O'Dowd, C. Botting, B. Precious, R. Shawcross, R. Randall (1999)
Novel modifications to the C-terminus of LTB that facilitate site-directed chemical coupling of antigens and the development of LTB as a carrier for mucosal vaccines.Vaccine, 17 11-12
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
(1995)
Structure and function of E. coli heat-labile enterotoxin and cholera toxin B pentamer
J. Pollock, P. Andersen (1997)
Predominant recognition of the ESAT-6 protein in the first phase of interferon with Mycobacterium bovis in cattleInfection and Immunity, 65
Maria, Sandkvist, T. Hirst, Michael, BAGDASARIAN't (1987)
Alterations at the carboxyl terminus change assembly and secretion properties of the B subunit of Escherichia coli heat-labile enterotoxinJournal of Bacteriology, 169
S. Liljeqvist, Stefan Ståhl, Christine Andréoni, Hans Binz, Mathias Uhlén, M. Murby (1997)
Fusions to the cholera toxin B subunit: influence on pentamerization and GM1 binding.Journal of immunological methods, 210 2
S. Clough, A. Bent (1998)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 16 6
W. Stemmer, A. Crameri, Kim Ha, T. Brennan, H. Heyneker (1995)
Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.Gene, 164 1
D. Becker, E. Kemper, J. Schell, R. Masterson (1992)
New plant binary vectors with selectable markers located proximal to the left T-DNA borderPlant Molecular Biology, 20
F. Sanger, S. Nicklen, A. Coulson (1977)
DNA sequencing with chain-terminating inhibitors.Proceedings of the National Academy of Sciences of the United States of America, 74 12
S. Aota, T. Gojobori, F. Ishibashi, T. Maruyama, T. Ikemura (1988)
Codon usage tabulated from the GenBank Genetic Sequence Data.Nucleic acids research, 16 Suppl
O. Emanuelsson, Henrik Nielsen, S. Brunak, G. Heijne (2000)
Predicting subcellular localization of proteins based on their N-terminal amino acid sequence.Journal of molecular biology, 300 4
(1997)
protects mice against Escherichia coli heat - labile enterotoxin ( LT ) : potatoes expressing a synthetic LTB gene
(1995)
Single - step assembly of a
L. Brandt, T. Oettinger, A. Holm, Å. Andersen, P. Andersen (1996)
Key epitopes on the ESAT-6 antigen recognized in mice during the recall of protective immunity to Mycobacterium tuberculosis.Journal of immunology, 157 8
M. Bagdasarian, M. Nagai, J. Frey, M. Bagdasarian (1999)
Immunogenicity of Actinobacillus ApxIA toxin epitopes fused to the E. coli heat-labile enterotoxin B subunit.Vaccine, 17 5
H. Mason, Tariq Haq, J. Clements, C. Arntzen (1998)
Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene.Vaccine, 16 13
M. Horwitz, Byong-Wha Lee, B. Dillon, G. Harth (1995)
Protective immunity against tuberculosis induced by vaccination with major extracellular proteins of Mycobacterium tuberculosis.Proceedings of the National Academy of Sciences of the United States of America, 92
E. Jagusztyn-Krynicka, J. Clark-Curtiss, R. Curtiss (1993)
Escherichia coli heat-labile toxin subunit B fusions with Streptococcus sobrinus antigens expressed by Salmonella typhimurium oral vaccine strains: importance of the linker for antigenicity and biological activities of the hybrid proteinsInfection and Immunity, 61
John Clements (1990)
Construction of a nontoxic fusion peptide for immunization against Escherichia coli strains that produce heat-labile and heat-stable enterotoxinsInfection and Immunity, 58
M. Ljpscombe, I. Charles, M. Roberts, G. Dougan, J. Tite, N. Fairweather (1991)
Intranasal immunization using the B subunit of the Escherichia coli heat‐labile toxin fused to an epitope of the Bordetella pertussis P.69 antigenMolecular Microbiology, 5
Seon-Kap Hwang, Young Kim (2000)
A Simple and Reliable Method for Preparation of Cross-Contamination-Free Plant Genomic DNA for PCR-Based Detection of TransgenesJournal of Biochemistry and Molecular Biology, 33
Henrik Nielsen, J. Engelbrecht, S. Brunak (1997)
SHORT COMMUNICATION Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites
Tariq Haq, H. Mason, J. Clements, CJ Arntzen (1995)
Oral immunization with a recombinant bacterial antigen produced in transgenic plantsScience, 268
Transgenic plants are potentially safe and inexpensive vehicles to produce and mucosally deliver protective antigens. However, the application of this technology is limited by the poor response of the immune system to non-particulate, subunit vaccines. Co-delivery of therapeutic proteins with carrier proteins could increase the effectiveness of the antigen. This paper reports the ability of transgenic Arabidopsis thaliana plants to produce a fusion protein consisting of the B subunit of the Escherichia coli heat-labile enterotoxin and a 6 kDa tuberculosis antigen, the early secretory antigenic target ESAT-6. Both components of the fusion protein were detected using GM1-ganglioside-dependent enzyme-linked immunosorbant assay. This suggested the fusion protein retained both its native antigenicity and the ability to form pentamers.
Plant Cell Reports – Springer Journals
Published: Feb 1, 2004
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