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C. Clancy, Y. Rudy (1999)
Linking a genetic defect to its cellular phenotype in a cardiac arrhythmiaNature, 400
H. Daniel (2004)
Molecular and integrative physiology of intestinal peptide transport.Annual review of physiology, 66
T. Terada, Kyoko Sawada, H. Saito, Y. Hashimoto, K. Inui (1999)
Functional characteristics of basolateral peptide transporter in the human intestinal cell line Caco-2.American journal of physiology. Gastrointestinal and liver physiology, 276 6
D. Thwaites, C. Brown, B. Hirst, N. Simmons (1993)
H(+)-coupled dipeptide (glycylsarcosine) transport across apical and basal borders of human intestinal Caco-2 cell monolayers display distinctive characteristics.Biochimica et biophysica acta, 1151 2
Yuji Sakurai, H. Motohashi, Harumasa Ueo, S. Masuda, H. Saito, M. Okuda, Noriko Mori, Motokazu Matsuura, T. Doi, A. Fukatsu, O. Ogawa, K. Inui (2004)
Expression Levels of Renal Organic Anion Transporters (OATs) and Their Correlation with Anionic Drug Excretion in Patients with Renal DiseasesPharmaceutical Research, 21
P. Vachon, J. Beaulieu (1992)
Transient mosaic patterns of morphological and functional differentiation in the Caco-2 cell line.Gastroenterology, 103 2
K Sawada, T Terada, H Saito, K Inui (2001)
Distinct transport characteristics of basolateral peptide transporters between MDCK and Caco-2 cellsPflugers Arch, 443
S. Matsuoka, N. Sarai, S. Kuratomi, K. Ono, A. Noma (2003)
Role of individual ionic current systems in ventricular cells hypothesized by a model study.The Japanese journal of physiology, 53 2
Kennedy D., Leibach F., G. V, T. D. (2002)
Optimal absorptive transport of the dipeptide glycylsarcosine is dependent on functional Na+/H+ exchange activityPflügers Archiv, 445
Xiaoyue Pan, T. Terada, Megumi Irie, H. Saito, K. Inui (2002)
Diurnal rhythm of H+-peptide cotransporter in rat small intestine.American journal of physiology. Gastrointestinal and liver physiology, 283 1
X. Chu, G. Sánchez-Castaño, K. Higaki, D. Oh, C. Hsu, G. Amidon (2001)
Correlation between epithelial cell permeability of cephalexin and expression of intestinal oligopeptide transporter.The Journal of pharmacology and experimental therapeutics, 299 2
M. Thamotharan, S. Bawani, Xiaodong Zhou, S. Adibi (1999)
Hormonal regulation of oligopeptide transporter Pept-1 in a human intestinal cell line.American journal of physiology. Cell physiology, 276 4
H. Saito, K. Inui (1993)
Dipeptide transporters in apical and basolateral membranes of the human intestinal cell line Caco-2.The American journal of physiology, 265 2 Pt 1
Megumi Irie, T. Terada, M. Okuda, K. Inui (2004)
Efflux properties of basolateral peptide transporter in human intestinal cell line Caco-2Pflügers Archiv, 449
A. Hoffmann, A. Levchenko, M. Scott, D. Baltimore (2002)
The IκB-NF-κB Signaling Module: Temporal Control and Selective Gene ActivationScience, 298
M. Buyse, F. Berlioz, S. Guilmeau, A. Tsocas, T. Voisin, G. Péranzi, D. Merlin, M. Laburthe, M. Lewin, C. Rozé, A. Bado (2001)
PepT1-mediated epithelial transport of dipeptides and cephalexin is enhanced by luminal leptin in the small intestine.The Journal of clinical investigation, 108 10
H. Daniel, G. Kottra (2004)
The proton oligopeptide cotransporter family SLC15 in physiology and pharmacologyPflügers Archiv, 447
S. Adibi (2003)
Regulation of expression of the intestinal oligopeptide transporter (Pept-1) in health and disease.American journal of physiology. Gastrointestinal and liver physiology, 285 5
Megumi Irie, T. Terada, Kyoko Sawada, H. Saito, K. Inui (2001)
Recognition and transport characteristics of nonpeptidic compounds by basolateral peptide transporter in Caco-2 cells.The Journal of pharmacology and experimental therapeutics, 298 2
T. Terada, K. Inui (2004)
Peptide transporters: structure, function, regulation and application for drug delivery.Current drug metabolism, 5 1
K. Naruhashi, Y. Sai, I. Tamai, Nagao Suzuki, A. Tsuji (2002)
PepT1 mRNA Expression Is Induced by Starvation and Its Level Correlates with Absorptive Transport of Cefadroxil Longitudinally in the Rat IntestinePharmaceutical Research, 19
S. Adibi (1997)
The oligopeptide transporter (Pept-1) in human intestine: biology and function.Gastroenterology, 113 1
H. Motohashi, Yuji Sakurai, H. Saito, Satohiro Masuda, Y. Urakami, Maki Goto, Atsushi Fukatsu, Osamu Ogawa, K. Inui (2002)
Gene expression levels and immunolocalization of organic ion transporters in the human kidney.Journal of the American Society of Nephrology : JASN, 13 4
D. Thwaites, C. Brown, B. Hirst, N. Simmons (1993)
Transepithelial glycylsarcosine transport in intestinal Caco-2 cells mediated by expression of H(+)-coupled carriers at both apical and basal membranes.The Journal of biological chemistry, 268 11
B. Schoeberl, Claudia Eichler-Jonsson, E. Gilles, G. Müller (2002)
Computational modeling of the dynamics of the MAP kinase cascade activated by surface and internalized EGF receptorsNature Biotechnology, 20
T. Hashida, S. Masuda, S. Uemoto, H. Saito, Koichi Tanaka, K. Inui (2001)
Pharmacokinetic and prognostic significance of intestinal MDR1 expression in recipients of living‐donor liver transplantationClinical Pharmacology & Therapeutics, 69
C. Yang, A. Dantzig, C. Pidgeon (1999)
Intestinal Peptide Transport Systems and Oral Drug AvailabilityPharmaceutical Research, 16
F. Leibach, Vadivel Ganapathy (1996)
Peptide transporters in the intestine and the kidney.Annual review of nutrition, 16
K. Inui, M. Yamamoto, H. Saito (1992)
Transepithelial transport of oral cephalosporins by monolayers of intestinal epithelial cell line Caco-2: specific transport systems in apical and basolateral membranes.The Journal of pharmacology and experimental therapeutics, 261 1
H+-coupled peptide transporter 1 (PEPT1) and the basolateral peptide transporter mediate the absorption of small peptides and peptide-like drugs in the small intestine. Recently, we constructed a mathematical model to simulate glycylsarcosine (Gly-Sar) transport in Caco-2 cells. In this study, we attempted to adjust our model to a change in the expression level of PEPT1. To obtain cell lines expressing PEPT1 at different levels, recloning of Caco-2 cells was performed, and nine clones were isolated. Compared with parental cells, clones 1 and 9 exhibited the lowest and the highest levels of [14C]Gly-Sar uptake from the apical side, respectively, whereas activities of the basolateral peptide transporter were comparable. Kinetic analysis demonstrated that the difference in the activity of PEPT1 was accounted by variations in V max. Moreover, PEPT1 mRNA level was positively related to the activity of [14C]Gly-Sar uptake (r=0.55). Based on these findings, the V max value of PEPT1 was defined as a variable using the amount of PEPT1 mRNA as an index of the expression level. With this improved model, Gly-Sar transport in clones 1 and 9 was well-predicted, suggesting that our model can simulate Gly-Sar transport in cells expressing PEPT1 at different levels.
Pflügers Archiv European Journal of Physiologyl of Physiology – Springer Journals
Published: Nov 10, 2005
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