221 48 48 1 1 Dr. D. Bingmann G. Kolde Institute of Physiology University of Münster Westring 6 D-4000 Münster Germany Institute of Cytobiology University of Münster Westring 6 D-4000 Münster Germany Summary For a detailed analysis of the oxygen supply of hippocampal slices, tissue PO 2 (P t,o 2 ) was recorded polarographically in the neural layers of thick and thin slice preparations from the guinea pig. The experiments showed that the P t ,o 2 -gradients were extremely steep in the outer zones of vital slices. In an air equilibrated salt solution the surface PO 2 was reduced to less than 50% within ca. 25 μm. Minimum values were measured at a depth of ca. 150 μm. A rise of temperature lowered the oxygen supply in the deeper layers of the excised tissue. An elevation of the surface PO 2 hardly improved P t,o 2 in the deep structures, because the O 2 -consumption of the hippocampal slices increased with rising PO 2 .
Experimental Brain Research – Springer Journals
Published: Oct 1, 1982
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