Plant regeneration from callus culture of Cymbidium ensifolium var. misericors

Plant regeneration from callus culture of Cymbidium ensifolium var. misericors Totipotent calli of Cymbidium ensifolium var. misericors, a locally grown orchid of high commercial value, were induced from sections of pseudobulbs, rhizomes and roots of seed-derived plantlets on 1/2-strength Murashige and Skoog medium plus 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l thiadiazuron. The calli could be maintained by subculturing in the same medium. The calli could be induced to develop further along one of three distinct morphogeneic routes: (1) production of rhizomes, (2) production of shoot buds, or (3) development of granular embryoids. Efficient mass propagation was possible via rhizome proliferation and embryoid formation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Plant regeneration from callus culture of Cymbidium ensifolium var. misericors

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Publisher
Springer-Verlag
Copyright
Copyright © 1998 by Springer-Verlag Berlin Heidelberg
Subject
Legacy
ISSN
0721-7714
eISSN
1432-203X
D.O.I.
10.1007/s002990050387
Publisher site
See Article on Publisher Site

Abstract

Totipotent calli of Cymbidium ensifolium var. misericors, a locally grown orchid of high commercial value, were induced from sections of pseudobulbs, rhizomes and roots of seed-derived plantlets on 1/2-strength Murashige and Skoog medium plus 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l thiadiazuron. The calli could be maintained by subculturing in the same medium. The calli could be induced to develop further along one of three distinct morphogeneic routes: (1) production of rhizomes, (2) production of shoot buds, or (3) development of granular embryoids. Efficient mass propagation was possible via rhizome proliferation and embryoid formation.

Journal

Plant Cell ReportsSpringer Journals

Published: Feb 1, 1998

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