Phenolic composition of rhubarb

Phenolic composition of rhubarb Extracts of different parts (leaves, petioles and rhizomes) of domestic garden rhubarb (hybrids of Rheum rhabarbarum L. and Rheum rhaponticum L.) were investigated for their content of phenolic ingredients. Two stilbenes (trans-rhapontigenin, trans-desoxyrhapontigenin), five stilbene glycosides (trans-rhaponticin, cis- and trans-desoxyrhaponticin, trans-resveratrol-4′-O-β-d-glucopyranoside, trans-piceatannol-3′-O-β-d-glucopyranoside) and seven flavonoids [rutin, quercetin-3-O-glucuronide, isovitexin, 6,8-di-C-β-d-glucosylapigenin, 6-C-β-d-glucosyl-8-C-β-d-arabinosylapigenin (schaftoside), 6-C-β-d-arabinosyl-8-C-β-d-glycosylapigenin (isoschaftoside), (+)-catechin] were unequivocally established. Separation was done in two steps. Multilayer countercurrent chromatography was applied to separate different extracts of plant material. Preparative HPLC was then used to obtain pure substances. The purity and identity of isolated compounds was confirmed by different NMR experiments, HR-MS and HPLC-DAD analysis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Food Research and Technology Springer Journals

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Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Chemistry; Forestry; Agriculture; Biotechnology; Analytical Chemistry ; Food Science
ISSN
1438-2377
eISSN
1438-2385
DOI
10.1007/s00217-008-0922-y
Publisher site
See Article on Publisher Site

Abstract

Extracts of different parts (leaves, petioles and rhizomes) of domestic garden rhubarb (hybrids of Rheum rhabarbarum L. and Rheum rhaponticum L.) were investigated for their content of phenolic ingredients. Two stilbenes (trans-rhapontigenin, trans-desoxyrhapontigenin), five stilbene glycosides (trans-rhaponticin, cis- and trans-desoxyrhaponticin, trans-resveratrol-4′-O-β-d-glucopyranoside, trans-piceatannol-3′-O-β-d-glucopyranoside) and seven flavonoids [rutin, quercetin-3-O-glucuronide, isovitexin, 6,8-di-C-β-d-glucosylapigenin, 6-C-β-d-glucosyl-8-C-β-d-arabinosylapigenin (schaftoside), 6-C-β-d-arabinosyl-8-C-β-d-glycosylapigenin (isoschaftoside), (+)-catechin] were unequivocally established. Separation was done in two steps. Multilayer countercurrent chromatography was applied to separate different extracts of plant material. Preparative HPLC was then used to obtain pure substances. The purity and identity of isolated compounds was confirmed by different NMR experiments, HR-MS and HPLC-DAD analysis.

Journal

European Food Research and TechnologySpringer Journals

Published: Jul 23, 2008

References

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