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Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase

Pearl millet transformation system using the positive selectable marker gene phosphomannose... Fertile transgenic pearl millet plants expressing a phosphomannose isomerase (PMI) transgene under control of the maize ubiquitin constitutive promoter were obtained using the transformation system described here. Proliferating immature zygotic embryos were used as target tissue for bombardment using a particle inflow gun. Different culture and selection strategies were assessed in order to obtain an optimised mannose selection protocol. Stable integration of the man A gene into the genome of pearl millet was confirmed by PCR and Southern blot analysis. Stable integration of the man A transgene into the genome of pearl millet was demonstrated in T 1 and T 2 progeny of two independent transformation events with no more than four to ten copies of the transgene. Similar to results obtained from previous studies with maize and wheat, the man A gene was shown to be a superior selectable marker gene for improving transformation efficiencies when compared to antibiotic or herbicide selectable marker genes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Pearl millet transformation system using the positive selectable marker gene phosphomannose isomerase

Plant Cell Reports , Volume 22 (9) – Apr 1, 2004

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References (21)

Publisher
Springer Journals
Copyright
Copyright © 2004 by Springer-Verlag
Subject
LifeSciences
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-003-0746-y
pmid
14727053
Publisher site
See Article on Publisher Site

Abstract

Fertile transgenic pearl millet plants expressing a phosphomannose isomerase (PMI) transgene under control of the maize ubiquitin constitutive promoter were obtained using the transformation system described here. Proliferating immature zygotic embryos were used as target tissue for bombardment using a particle inflow gun. Different culture and selection strategies were assessed in order to obtain an optimised mannose selection protocol. Stable integration of the man A gene into the genome of pearl millet was confirmed by PCR and Southern blot analysis. Stable integration of the man A transgene into the genome of pearl millet was demonstrated in T 1 and T 2 progeny of two independent transformation events with no more than four to ten copies of the transgene. Similar to results obtained from previous studies with maize and wheat, the man A gene was shown to be a superior selectable marker gene for improving transformation efficiencies when compared to antibiotic or herbicide selectable marker genes.

Journal

Plant Cell ReportsSpringer Journals

Published: Apr 1, 2004

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