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Obtaining of water soluble microbial melanin and study of its some properties

Obtaining of water soluble microbial melanin and study of its some properties The effective sorption method for melanin isolation and purification from fermentation solutions of Bacillus thuringiensis serovar galleriae K1 has been elaborated, the principle process flowsheet is presented. The identification of obtained pigment with the samples of natural and synthetic melanin was done by IR-spectroscopy, and the intensity ratio of optical absorption at 650 and 500 nm allows to refer the isolated melanin to eumelanin class. By thermal treatment it was determined, that melanin’s amorphous sediment is steady at up to 120°C) the concentration of paramagnetic centers being changed from 0.053 × 1018 spin/g (48°C) to 0.25 × 1018 spin/g (120°C). The rising of the temperature of treatment up to 210°C brings to substantial increase of the concentration of unpaired electrons, and at 280°C its sharp growth is observed. At 350°C growth stops, then sharp decrease is observed. The obtained results were confirmed by methods of IR spectroscopy and derivatographic analysis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Obtaining of water soluble microbial melanin and study of its some properties

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References (11)

Publisher
Springer Journals
Copyright
Copyright © 2010 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Medical Microbiology; Microbiology; Biochemistry, general
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683811050024
Publisher site
See Article on Publisher Site

Abstract

The effective sorption method for melanin isolation and purification from fermentation solutions of Bacillus thuringiensis serovar galleriae K1 has been elaborated, the principle process flowsheet is presented. The identification of obtained pigment with the samples of natural and synthetic melanin was done by IR-spectroscopy, and the intensity ratio of optical absorption at 650 and 500 nm allows to refer the isolated melanin to eumelanin class. By thermal treatment it was determined, that melanin’s amorphous sediment is steady at up to 120°C) the concentration of paramagnetic centers being changed from 0.053 × 1018 spin/g (48°C) to 0.25 × 1018 spin/g (120°C). The rising of the temperature of treatment up to 210°C brings to substantial increase of the concentration of unpaired electrons, and at 280°C its sharp growth is observed. At 350°C growth stops, then sharp decrease is observed. The obtained results were confirmed by methods of IR spectroscopy and derivatographic analysis.

Journal

Applied Biochemistry and MicrobiologySpringer Journals

Published: Sep 7, 2011

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