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Nicotiana sylvestris calcineurin B-like protein NsylCBL10 enhances salt tolerance in transgenic Arabidopsis

Nicotiana sylvestris calcineurin B-like protein NsylCBL10 enhances salt tolerance in transgenic... The calcineurin B-like (CBL) proteins represent a unique group of plant calcium sensors and play an important role in regulating the response of a plant cell to the stress. Although many studies have been made in Arabidopsis ( Arabidopsis thaliana ), rice ( Oryza sativa ) and poplar ( Populus trichocarpa ), the characterization and elucidation of the functions of CBLs in tobacco have not yet been reported. In this study, NsylCBL10 , a CBL gene showing higher similarities to other CBL10 genes, was cloned from Nicotiana sylvestris . NsylCBL10 is expressed in most of the tobacco tissues, and the protein targets to the plasma membrane specifically. Over-expression of NsylCBL10 enhanced the salt tolerance of Arabidopsis wild type plants greatly, and rescued the high-salt-sensitive phenotype of Arabidopsis cbl10 mutant. The analysis of ion content indicated that over-expressing NsylCBL10 in plants is able to maintain a lower Na + /K + ratio in roots and higher Na + /K + ratio in shoots, compared with cbl10 mutant. The results suggest that NsylCBL10 might play an important role in response to high salinity stress in N . sylvestris , by keeping a better ionic homeostasis to reduce the damage of toxic ion to the plant cell. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Nicotiana sylvestris calcineurin B-like protein NsylCBL10 enhances salt tolerance in transgenic Arabidopsis

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References (42)

Publisher
Springer Journals
Copyright
Copyright © 2015 by Springer-Verlag Berlin Heidelberg
Subject
Life Sciences; Plant Sciences; Cell Biology; Biotechnology; Plant Biochemistry
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-015-1851-4
pmid
26318216
Publisher site
See Article on Publisher Site

Abstract

The calcineurin B-like (CBL) proteins represent a unique group of plant calcium sensors and play an important role in regulating the response of a plant cell to the stress. Although many studies have been made in Arabidopsis ( Arabidopsis thaliana ), rice ( Oryza sativa ) and poplar ( Populus trichocarpa ), the characterization and elucidation of the functions of CBLs in tobacco have not yet been reported. In this study, NsylCBL10 , a CBL gene showing higher similarities to other CBL10 genes, was cloned from Nicotiana sylvestris . NsylCBL10 is expressed in most of the tobacco tissues, and the protein targets to the plasma membrane specifically. Over-expression of NsylCBL10 enhanced the salt tolerance of Arabidopsis wild type plants greatly, and rescued the high-salt-sensitive phenotype of Arabidopsis cbl10 mutant. The analysis of ion content indicated that over-expressing NsylCBL10 in plants is able to maintain a lower Na + /K + ratio in roots and higher Na + /K + ratio in shoots, compared with cbl10 mutant. The results suggest that NsylCBL10 might play an important role in response to high salinity stress in N . sylvestris , by keeping a better ionic homeostasis to reduce the damage of toxic ion to the plant cell.

Journal

Plant Cell ReportsSpringer Journals

Published: Dec 1, 2015

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