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C. Dogovski, J. Pi, A. Pittard (2003)
Putative Interhelical Interactions within the PheP Protein Revealed by Second-Site Suppressor AnalysisJournal of Bacteriology, 185
A. Bogan, K. Thorn (1998)
Anatomy of hot spots in protein interfaces.Journal of molecular biology, 280 1
J. Pięta, De, Boer, R. Crossley, L. Rothfield (1992)
Roles of MinC and MinD in the site-specific septation block mediated by the MinCDE system of Escherichia coliJournal of Bacteriology, 174
P. Boer, R. Crossley, L. Rothfield (1989)
A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coliCell, 56
Zonglin Hu, J. Lutkenhaus (2000)
Analysis of MinC Reveals Two Independent Domains Involved in Interaction with MinD and FtsZJournal of Bacteriology, 182
P. Boer, R. Crossley, L. Rothfield (1988)
Isolation and properties of minB, a complex genetic locus involved in correct placement of the division site in Escherichia coliJournal of Bacteriology, 170
Mee Kim, Y. Kang (1999)
Positional preference of proline in α‐helicesProtein Science, 8
Nelson Eng, J. Szeto, S. Acharya, D. Tessier, J. Dillon (2006)
The C-terminus of MinE from Neisseria gonorrhoeae acts as a topological specificity factor by modulating MinD activity in bacterial cell division.Research in microbiology, 157 4
Zonglin Hu, Amit Mukherjee, S. Pichoff, J. Lutkenhaus (1999)
The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization.Proceedings of the National Academy of Sciences of the United States of America, 96 26
E. Mulder, L. Conrad, Woldringh, F. Tétart, JEAN-PIERRE, BOUCHt (1992)
New minC mutations suggest different interactions of the same region of division inhibitor MinC with proteins specific for minD and dicB coinhibition pathwaysJournal of Bacteriology, 174
L. Rothfield, A. Taghbalout, Y. Shih (2005)
Spatial control of bacterial division-site placementNature Reviews Microbiology, 3
J. Szeto, S. Ramirez‐Arcos, Claude Raymond, L. Hicks, C. Kay, J. Dillon (2001)
Gonococcal MinD Affects Cell Division inNeisseria gonorrhoeae and Escherichia coli and Exhibits a Novel Self-InteractionJournal of Bacteriology, 183
C. Labie, F. Bouche, J. Bouché (1990)
Minicell-forming mutants of Escherichia coli: suppression of both DicB- and MinD-dependent division inhibition by inactivation of the minC gene productJournal of Bacteriology, 172
S. Ramirez‐Arcos, V. Greco, H. Douglas, Daniel Tessier, D. Fan, J. Szeto, J. Wang, J-a.r. Dillon (2004)
Conserved Glycines in the C Terminus of MinC Proteins Are Implicated in Their Functionality as Cell Division InhibitorsJournal of Bacteriology, 186
Anton Persikov, J. Ramshaw, A. Kirkpatrick, B. Brodsky (2005)
Electrostatic interactions involving lysine make major contributions to collagen triple-helix stability.Biochemistry, 44 5
S. Cordell, Rebecca Anderson, J. Löwe (2001)
Crystal structure of the bacterial cell division inhibitor MinCThe EMBO Journal, 20
S. Ramirez-Arcos, J. Szeto, Terrance Beveridge, Charles Victor, Finola Francis, J Dillon (2001)
Deletion of the cell-division inhibitor MinC results in lysis of Neisseria gonorrhoeae.Microbiology, 147 Pt 1
Jeffrey Miller (1972)
Experiments in molecular genetics
Rudresh, R. Jain, V. Dani, A. Mitra, S. Srivastava, S. Sarma, R. Varadarajan, S. Ramakumar (2002)
Structural consequences of replacement of an alpha-helical Pro residue in Escherichia coli thioredoxin.Protein engineering, 15 8
F. Picard, J. Dillon (1989)
Biochemical and genetic studies with arginine and proline auxotrophs of Neisseria gonorrhoeae.Canadian journal of microbiology, 35 12
G. Lallo, L. Castagnoli, P. Ghelardini, L. Paolozzi (2001)
A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli.Microbiology, 147 Pt 6
H. Salimnia, A. Radia, S. Bernatchez, T. Beveridge, J. Dillon (2000)
Characterization of the ftsZ cell division gene of Neisseria gonorrhoeae: expression in Escherichia coli and N. gonorrhoeaeArchives of Microbiology, 173
While bacterial cell division has been widely studied in rod-shaped bacteria, the mechanism of cell division in round (coccal) bacteria remains largely enigmatic. In the present study, interaction between the cell division inhibitor MinC from Neisseria gonorrhoeae (MinC Ng ) and the gonococcal cell division proteins MinD Ng and FtsZ Ng are demonstrated. Protein truncation and site-directed mutagenic approaches determined which N-terminal residues were essential for cell division inhibition by MinC Ng using cell morphology as an indicator of protein functionality. Truncation from or mutation at the 13th amino acid of the N terminus of MinC Ng resulted in loss of protein function. Bioinformatic analyses predicted that point mutations of L35P and L68P would affect the α-helical conformation of the protein and we experimentally showed that these mutations alter the functionality of MinC Ng . The bacterial two-hybrid system showed that interaction of MinC Ng with FtsZ Ng is abrogated upon truncation of 13 N-terminal residues while MinC Ng -MinD Ng interaction or MinC Ng homodimerization is unaffected. These data confirm interactions among gonococcal cell division proteins and determine the necessity of the 13th amino acid for MinC Ng function.
Archives of Microbiology – Springer Journals
Published: Jun 1, 2007
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