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Mutation in the lysA gene impairs the symbiotic properties of Mesorhizobium ciceri

Mutation in the lysA gene impairs the symbiotic properties of Mesorhizobium ciceri A Tn5-induced mutant of Mesorhizobium ciceri , TL28, requiring the amino acid lysine for growth on minimal medium was isolated and characterized. The Tn5 insertion in the mutant strain TL28 was located on a 6.8-kb Eco RI fragment of the chromosomal DNA. Complementation analysis with cloned DNA indicated that 1.269 kb of DNA of the 6.8-kb Eco RI fragment restored the wild-type phenotype of the lysine-requiring mutant. This region was further characterized by DNA sequence analysis and was shown to contain a coding sequence homologous to lysA gene of different bacteria. The lys − mutant TL28 was unable to elicit development of effective nodules on the roots of Cicer arietinum L. There was no detectable level of lysine in the root exudates of chickpea. However, addition of lysine to the plant growth medium restored the ability of the mutant to produce effective nodules with nitrogen fixation ability on the roots of C. arietinum . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Mutation in the lysA gene impairs the symbiotic properties of Mesorhizobium ciceri

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References (39)

Publisher
Springer Journals
Copyright
Copyright © 2010 by Springer-Verlag
Subject
Life Sciences; Ecology; Biotechnology; Biochemistry, general; Cell Biology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/s00203-009-0527-2
pmid
20020102
Publisher site
See Article on Publisher Site

Abstract

A Tn5-induced mutant of Mesorhizobium ciceri , TL28, requiring the amino acid lysine for growth on minimal medium was isolated and characterized. The Tn5 insertion in the mutant strain TL28 was located on a 6.8-kb Eco RI fragment of the chromosomal DNA. Complementation analysis with cloned DNA indicated that 1.269 kb of DNA of the 6.8-kb Eco RI fragment restored the wild-type phenotype of the lysine-requiring mutant. This region was further characterized by DNA sequence analysis and was shown to contain a coding sequence homologous to lysA gene of different bacteria. The lys − mutant TL28 was unable to elicit development of effective nodules on the roots of Cicer arietinum L. There was no detectable level of lysine in the root exudates of chickpea. However, addition of lysine to the plant growth medium restored the ability of the mutant to produce effective nodules with nitrogen fixation ability on the roots of C. arietinum .

Journal

Archives of MicrobiologySpringer Journals

Published: Jan 1, 2010

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