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122 89 89 4 4 S. T. Berry R. J. Allen S. R. Barnes P. D. S. Caligari ZENECA Seeds, Plant Biotechnology Jealott's Hill Research Station RG12 6EY Bracknell Berkshire UK ZENECA Seeds-SES Industriepark 15 B-3300 Tienen Belgium Department of Agricultural Botany School of Plant Sciences, University of Reading Whiteknights PO Box 221 RG6 2AS Reading Berkshire UK Abstract cDNA and Pst I genomic clones have been used to assess levels of restriction fragment length polymorphism (RFLP) in Helianthus annuus and to determine the inter-relationships between a diverse set of 24 inbred lines. Of the cDNA clones screened 45% were useful as RFLP probes, compared to less than 20% from the Pst I library, which showed high levels of redundancy for high copy sequences. Fifty-seven low-copy DNA probes (23 Pst I and 34 cDNA clones) were used to fingerprint 12 maintainer (B) lines and 12 restorer (R) lines. The average number of RFLP variants per probe was found to be 3.2, with a mean polymorphic index of 0.49, indicating that high levels of nuclear DNA polymorphism are to be found in cultivated sunflower. Cluster and principal coordinate analysis of the fingerprinting data clearly separated the maintainer and restorer lines, but there was a degree of association between 2 unbranched R-lines and the B-line germ plasm pool.
TAG Theoretical and Applied Genetics – Springer Journals
Published: Oct 1, 1994
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