At present, small number imprinted genes have been reported in cattle compare to human and mouse. Maternally expressed gene 8 (Meg8), a non-coding gene, have been identified maternally expressed in mouse and sheep, but its sequences and imprinting status have not been established in cattle. In this study, the full-length of cattle Meg8 cDNA sequence was obtained by reverse transcript polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) method, which has a high homology in exons sequences with the corresponding region of sheep Meg8. The isolation of cDNA sequence showed the presence of multiple splice variants in cattle Meg8 gene. The Meg8 was found to be expressed in all adult examined tissues, including heart, liver, spleen, lung, kidney, brain, subcutaneous fat and skeletal muscle. A single nucleotide polymorphism (SNP) was identified in exon 6 by Single-Strand Conformation Polymorphism (SSCP), and used to distinguish between monoallelic and biallelic expression in cattle tissues. The expression analysis of Meg8 in a heterozygous cattle showed that only one parental allele was expressed in all examined tissues, suggesting that Meg8 is imprinted in cattle.
Russian Journal of Genetics – Springer Journals
Published: Aug 19, 2011
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera