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Molecular Cloning and Tissue Distribution of SF-1-related Orphan Receptors During Sexual Maturation in Female Goldfish

Molecular Cloning and Tissue Distribution of SF-1-related Orphan Receptors During Sexual... The steroidogenic factor (SF)-1 gene is one of a number of orphan nuclear receptors, which is a key transcriptional regulator in vertebrate reproduction. We have isolated the SF-1 homologue cDNA from the goldfish pituitary and designed primers for SF-1 on the basis of the highly conserved regions of various known SF-1 superfamily genes. SF-1 cDNA contained 1,948 nucleotides including an open reading frame predicted to encode a protein of 503 amino acids. The distribution pattern of SF-1 in a variety of tissues during sexual maturation in female goldfish was also examined by RT-PCR. Significant variations in the relative expression of SF-1 were observed in different tissues in immature and mature female goldfish. SF-1 transcript in pituitary was significantly higher than other tissues tested in immature and mature female goldfish. Lower expression of SF-1 was observed in the liver but was not detected in brain and ovary of the immature female goldfish. Presence of SF-1 was the predominant expression in the pituitary and brain of mature female goldfish. Also, in the mature female goldfish, a weak transcript was detected in liver and ovary. Interestingly, RT-PCR analysis revealed that the expression of SF-1 became higher in the brain and weaker in the liver in maturing female goldfish. Thus, SF-1 may be regulated in goldfish brain and/or liver. Thus is also tissue-specific distribution of SF-1 during sexual maturation in female goldfish. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Letters Springer Journals

Molecular Cloning and Tissue Distribution of SF-1-related Orphan Receptors During Sexual Maturation in Female Goldfish

Choi, Cheol; Habibi, Hamid
Biotechnology Letters , Volume 27 (17) – Jun 27, 2005
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References (26)

Publisher
Springer Journals
Copyright
Copyright © 2005 by Springer
Subject
Chemistry; Biotechnology; Applied Microbiology; Biochemistry, general; Microbiology; Bioorganic Chemistry
ISSN
0141-5492
eISSN
1573-6776
DOI
10.1007/s10529-005-0220-8
pmid
16215826
Publisher site
See Article on Publisher Site

Abstract

The steroidogenic factor (SF)-1 gene is one of a number of orphan nuclear receptors, which is a key transcriptional regulator in vertebrate reproduction. We have isolated the SF-1 homologue cDNA from the goldfish pituitary and designed primers for SF-1 on the basis of the highly conserved regions of various known SF-1 superfamily genes. SF-1 cDNA contained 1,948 nucleotides including an open reading frame predicted to encode a protein of 503 amino acids. The distribution pattern of SF-1 in a variety of tissues during sexual maturation in female goldfish was also examined by RT-PCR. Significant variations in the relative expression of SF-1 were observed in different tissues in immature and mature female goldfish. SF-1 transcript in pituitary was significantly higher than other tissues tested in immature and mature female goldfish. Lower expression of SF-1 was observed in the liver but was not detected in brain and ovary of the immature female goldfish. Presence of SF-1 was the predominant expression in the pituitary and brain of mature female goldfish. Also, in the mature female goldfish, a weak transcript was detected in liver and ovary. Interestingly, RT-PCR analysis revealed that the expression of SF-1 became higher in the brain and weaker in the liver in maturing female goldfish. Thus, SF-1 may be regulated in goldfish brain and/or liver. Thus is also tissue-specific distribution of SF-1 during sexual maturation in female goldfish.

Journal

Biotechnology Letters – Springer Journals

Published: Jun 27, 2005

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