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Microbial biodiversity in a Malaysian oil field and a systematic comparison with oil reservoirs worldwide

Microbial biodiversity in a Malaysian oil field and a systematic comparison with oil reservoirs... Microbial diversity within formation water and oil from two compartments in Bokor oil reservoir from a Malaysian petroleum oil field was examined. A total of 1,056 16S rRNA gene clones were screened from each location by amplified ribosomal DNA restriction analysis. All samples were dominated by clones affiliated with Marinobacter , some novel Deferribacteraceae genera and various clones allied to the Methanococci. In addition, either Marinobacterium - or Pseudomonas -like operational taxonomic units were detected from either compartment. A systematic comparison with the existing pertinent studies was undertaken by analysing the microbial amplicons detected and the PCR primers used. The analyses demonstrated that bacterial communities were site specific, while Archaea co-occurred more frequently. Amplicons related to Marinobacter, Marinobacterium and Pseudomonas were detected in a number of the studies examined, suggesting they may be ubiquitous members in oil reservoirs. Further analysis of primers used in those studies suggested that most primer pairs had fairly broad but low matches across the bacterial and archaeal domains, while a minority had selective matches to certain taxa or low matches to all the microbial taxa tested. Thus, it indicated that primers may play an important role in determining which taxa would be detected. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Microbial biodiversity in a Malaysian oil field and a systematic comparison with oil reservoirs worldwide

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Publisher
Springer Journals
Copyright
Copyright © 2012 by Springer-Verlag
Subject
Life Sciences; Microbiology; Cell Biology; Ecology; Biotechnology; Biochemistry, general; Microbial Ecology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/s00203-012-0788-z
pmid
22245906
Publisher site
See Article on Publisher Site

Abstract

Microbial diversity within formation water and oil from two compartments in Bokor oil reservoir from a Malaysian petroleum oil field was examined. A total of 1,056 16S rRNA gene clones were screened from each location by amplified ribosomal DNA restriction analysis. All samples were dominated by clones affiliated with Marinobacter , some novel Deferribacteraceae genera and various clones allied to the Methanococci. In addition, either Marinobacterium - or Pseudomonas -like operational taxonomic units were detected from either compartment. A systematic comparison with the existing pertinent studies was undertaken by analysing the microbial amplicons detected and the PCR primers used. The analyses demonstrated that bacterial communities were site specific, while Archaea co-occurred more frequently. Amplicons related to Marinobacter, Marinobacterium and Pseudomonas were detected in a number of the studies examined, suggesting they may be ubiquitous members in oil reservoirs. Further analysis of primers used in those studies suggested that most primer pairs had fairly broad but low matches across the bacterial and archaeal domains, while a minority had selective matches to certain taxa or low matches to all the microbial taxa tested. Thus, it indicated that primers may play an important role in determining which taxa would be detected.

Journal

Archives of Microbiology – Springer Journals

Published: Jun 1, 2012

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