Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Microbial activities as a function of water depth in the Ligurian Sea: an autoradiographic study

Microbial activities as a function of water depth in the Ligurian Sea: an autoradiographic study 227 30 30 1 1 C. Peroni O. Lavarello Laboratorio per lo Studio della Contaminazione Radioattiva del Mare CNEN-EURATOM Fiascherino La Spezia Italy Abstract One of the main problems in marine microbiology is the assessment of the active microbial population and its distribution. The methods so far used are inadequate to provide reliable bacterial counts. An autoradiographic method is presented, based on 32 p-uptake by living bacterial cells which then imprint a radio-sensitive film. The number of spots counted on the imprinted films gives the concentration of heterotrophs present in the sample. By this method, high concentrations of metabolizing bacteria have been detected in the surface layers of the sea, indicating that the majority of bacterial cells, revealed by direct counts, are actually metabolizing. On the other hand, in layers below 150 to 200 m, the number of spots, i.e., bacteria, is negligible. Several factors may contribute to this situation; these are discussed briefly. Advantages and further applications of the described autoradiographic method are indicated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Marine Biology Springer Journals

Microbial activities as a function of water depth in the Ligurian Sea: an autoradiographic study

Peroni, C.; Lavarello, O.
Marine Biology , Volume 30 (1) – Apr 1, 1975
Download PDF
14 pages

Loading next page...
 
/lp/springer-journals/microbial-activities-as-a-function-of-water-depth-in-the-ligurian-sea-LWXLaRSoYR

References (28)

Publisher
Springer Journals
Copyright
Copyright © 1975 by Springer-Verlag
Subject
Life Sciences; Biomedicine general; Oceanography; Ecology; Microbiology; Zoology
ISSN
0025-3162
eISSN
1432-1793
DOI
10.1007/BF00393751
Publisher site
See Article on Publisher Site

Abstract

227 30 30 1 1 C. Peroni O. Lavarello Laboratorio per lo Studio della Contaminazione Radioattiva del Mare CNEN-EURATOM Fiascherino La Spezia Italy Abstract One of the main problems in marine microbiology is the assessment of the active microbial population and its distribution. The methods so far used are inadequate to provide reliable bacterial counts. An autoradiographic method is presented, based on 32 p-uptake by living bacterial cells which then imprint a radio-sensitive film. The number of spots counted on the imprinted films gives the concentration of heterotrophs present in the sample. By this method, high concentrations of metabolizing bacteria have been detected in the surface layers of the sea, indicating that the majority of bacterial cells, revealed by direct counts, are actually metabolizing. On the other hand, in layers below 150 to 200 m, the number of spots, i.e., bacteria, is negligible. Several factors may contribute to this situation; these are discussed briefly. Advantages and further applications of the described autoradiographic method are indicated.

Journal

Marine Biology – Springer Journals

Published: Apr 1, 1975

There are no references for this article.