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203 155 155 4 4 S. E. Lowe J. G. Zeikus Department of Biochemistry Michigan State University 48824 East Lansing MI USA Department of Microbiology and Public Health Michigan State University 48824 East Lansing MI USA the Michigan Biotechnology Institute 48909 Lansing MI USA Abstract The physiology and biochemistry of Sarcina ventriculi was studied in order to determine adaptations made by the organism to changes in environmental pH. The organism altered carbon and electron flow from acetate, formate and ethanol production at neutral pH, to predominantly ethanol production at pH 3.0. Increased levels of pyruvate dehydrogenase (relative to pyruvate decarboxylase) and acetaldehyde dehydrogenase occurred when the organism was grown at neutral pH, indicating the predominance of carbon flux through the oxidative branch of the pathway for pyruvate metabolism. When the organism was grown at acid pH, there was a significant increase in pyruvate decarboxylase levels and a decrease in acetaldehyde dehydrogenase, causing flux through the non-oxidative branch of the pathway. CO 2 reductase and formate dehydrogenase were not regulated as a function of growth pH. Pyruvate dehydrogenase possessed Michaelis-Menten kinetics for pyruvate with an apparent K m of 2.5 mM, whereas pyruvate decarboxylase exhibited sigmoidal kinetics, with a S 0.5 of 12.0 mM. Differences in total protein banding patterns from cells grown at pH extremes suggested that synthesis of pyruvate decarboxylase and other enzymes was in part responsible for metabolic regulation of the fermentation products formed.
Archives of Microbiology – Springer Journals
Published: Mar 1, 1991
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