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Metabolic flexibility of Thiobacillus A 2 during substrate transitions in the chemostat

Metabolic flexibility of Thiobacillus A 2 during substrate transitions in the chemostat 203 129 129 1 1 Jan C. Gottschal Arjan Pol J. Gijs Kuenen Laboratorium voor Microbiologie Rijksunivesiteit, Groningen Kerklaan 30 9751 NN Haren The Netherlands Department of Botany and Microbiology University College of Wales SY23 3DA Aberystwyth UK Laboratorium voor Microbiologie Katholieke Universiteit Toernooiveld Nijmegen The Netherlands Laboratorium voor Microbiologie Technische Hogeschool Julianalaan 67A 2628 BC Delft The Netherlands Abstract During autotrophic growth, cells of Thiobacillus A 2 retained a considerable capacity to oxidize various organic energy sources. Heterotrophically grown cultures, on the other hand, were completely devoid of the capacity to fix CO 2 via the Calvin cycle and to generate energy from thiosulfate. During transitions from organic media to inorganic thiosulfate-containing media in the chemostat, a long lag-phase was observed before energy generation, CO 2 fixation and, consequenctly, measurable growth occurred. This lag-phase was practically abolished if substrates were presentm at very low concentrations in the thiosulfate mineral medium which could be used as an energy source. The same result was obtained when the cells contained reserve material at the moment of the transition. During transitions from thiosulfate-limited growth to starvation, the -thiosulfate and the capacity to fix CO 2 decreased very slowly, after an initial short (± 4 h) increase of both enzyme systems. In contrast, these two metabolic functions were inactivated relatively rapidly in the presence of an oxidizable organic carbon and energy source. This process of inactivation was instantaneously stopped and reversed into rapid enzyme synthesis upon replacement of the organic substrate by thiosulfate. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Metabolic flexibility of Thiobacillus A 2 during substrate transitions in the chemostat

Gottschal, Jan; Pol, Arjan; Kuenen, J.
Archives of Microbiology , Volume 129 (1) – Mar 1, 1981
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References (28)

Publisher
Springer Journals
Copyright
Copyright © 1981 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00417173
Publisher site
See Article on Publisher Site

Abstract

203 129 129 1 1 Jan C. Gottschal Arjan Pol J. Gijs Kuenen Laboratorium voor Microbiologie Rijksunivesiteit, Groningen Kerklaan 30 9751 NN Haren The Netherlands Department of Botany and Microbiology University College of Wales SY23 3DA Aberystwyth UK Laboratorium voor Microbiologie Katholieke Universiteit Toernooiveld Nijmegen The Netherlands Laboratorium voor Microbiologie Technische Hogeschool Julianalaan 67A 2628 BC Delft The Netherlands Abstract During autotrophic growth, cells of Thiobacillus A 2 retained a considerable capacity to oxidize various organic energy sources. Heterotrophically grown cultures, on the other hand, were completely devoid of the capacity to fix CO 2 via the Calvin cycle and to generate energy from thiosulfate. During transitions from organic media to inorganic thiosulfate-containing media in the chemostat, a long lag-phase was observed before energy generation, CO 2 fixation and, consequenctly, measurable growth occurred. This lag-phase was practically abolished if substrates were presentm at very low concentrations in the thiosulfate mineral medium which could be used as an energy source. The same result was obtained when the cells contained reserve material at the moment of the transition. During transitions from thiosulfate-limited growth to starvation, the -thiosulfate and the capacity to fix CO 2 decreased very slowly, after an initial short (± 4 h) increase of both enzyme systems. In contrast, these two metabolic functions were inactivated relatively rapidly in the presence of an oxidizable organic carbon and energy source. This process of inactivation was instantaneously stopped and reversed into rapid enzyme synthesis upon replacement of the organic substrate by thiosulfate.

Journal

Archives of Microbiology – Springer Journals

Published: Mar 1, 1981

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