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Metabolic changes during sexual differentiation in Allomyces

Metabolic changes during sexual differentiation in Allomyces 203 57 57 3 3 Milos J. Kobr Gilbert Turian Laboratoire de Microbiologie, Institut de Botanique générale Université de Genève Geneve Switzeland Department of Biological Sciences Purdue University 47907 Lafayette Indiana USA Summary 1. Oxygen tension was found to be the primary factor inducing the sexual differentiation of the female colonies of Allomyces . 2. Vegetative colonies, or colonies at successive stages of induced sexual differentiation, were submitted to increasing periods of anoxia and analyzed for their content in some key metabolites. 3. Long periods of aeration altered the balance of the adenylates by favoring the build-up of ATP at the expense of ADP and AMP. During the same time, the FDP/F-6-P ratio was drastically lowered, indicating a shut-off of the PFK activity. 4. Anoxia was little effective in altering the balance of the adenylates or hexosephosphates in vegetative colonies. However, differentiating colonies were readily affected by anoxia: the balance of the adenylates shifted rapidly in favor of ADP and AMP, and the ratio between FDP and F-6-P rose markedly, pointing to an increased activity of the PFK. 5. From the different sensitivities to anoxia exhibited by ATP in vegetative vs. differentiated colonies, it was concluded that the sexual differentiation is accompanied by a shift of the respiratory pattern from a glycolytic to a predominantly oxidative one. This conclusion was further supported by the decrease of PFK endogenous activity during the differentiation; the ineffectiveness of anoxia in promoting PFK activity in vegetative colonies, contrasting with its ability to increase the FDP/F-6-P ratio in differentiating ones, points to the fact that glycolysis in vegetative colonies proceeds at nearly maximum capacity but substantially decreases during differentiation. 6. The concomitant changes undergone by the adenylates and the citrate on one hand, and by the FDP/F-6-P ratio on the other hand, are consistent with a regulation of the PFK activity by feed-back inhibition. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Metabolic changes during sexual differentiation in Allomyces

Kobr, Milos; Turian, Gilbert
Archives of Microbiology , Volume 57 (3) – Sep 1, 1967
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References (19)

Publisher
Springer Journals
Copyright
Copyright © 1967 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00405952
Publisher site
See Article on Publisher Site

Abstract

203 57 57 3 3 Milos J. Kobr Gilbert Turian Laboratoire de Microbiologie, Institut de Botanique générale Université de Genève Geneve Switzeland Department of Biological Sciences Purdue University 47907 Lafayette Indiana USA Summary 1. Oxygen tension was found to be the primary factor inducing the sexual differentiation of the female colonies of Allomyces . 2. Vegetative colonies, or colonies at successive stages of induced sexual differentiation, were submitted to increasing periods of anoxia and analyzed for their content in some key metabolites. 3. Long periods of aeration altered the balance of the adenylates by favoring the build-up of ATP at the expense of ADP and AMP. During the same time, the FDP/F-6-P ratio was drastically lowered, indicating a shut-off of the PFK activity. 4. Anoxia was little effective in altering the balance of the adenylates or hexosephosphates in vegetative colonies. However, differentiating colonies were readily affected by anoxia: the balance of the adenylates shifted rapidly in favor of ADP and AMP, and the ratio between FDP and F-6-P rose markedly, pointing to an increased activity of the PFK. 5. From the different sensitivities to anoxia exhibited by ATP in vegetative vs. differentiated colonies, it was concluded that the sexual differentiation is accompanied by a shift of the respiratory pattern from a glycolytic to a predominantly oxidative one. This conclusion was further supported by the decrease of PFK endogenous activity during the differentiation; the ineffectiveness of anoxia in promoting PFK activity in vegetative colonies, contrasting with its ability to increase the FDP/F-6-P ratio in differentiating ones, points to the fact that glycolysis in vegetative colonies proceeds at nearly maximum capacity but substantially decreases during differentiation. 6. The concomitant changes undergone by the adenylates and the citrate on one hand, and by the FDP/F-6-P ratio on the other hand, are consistent with a regulation of the PFK activity by feed-back inhibition.

Journal

Archives of Microbiology – Springer Journals

Published: Sep 1, 1967

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