Mechanism of action of nitrous oxide gas applied as a polyploidizing agent during meiosis in lilies

Mechanism of action of nitrous oxide gas applied as a polyploidizing agent during meiosis in lilies Nitrous oxide gas (N2O) can be used to produce polyploid plants, but the mechanism of action is unknown. The actin and microtubule cytoskeleton was observed in N2O-treated microsporocytes of Lilium spp ‘Asiatic hybrid lilies’ using fluorescence microscopy after staining with DAPI, FITC-conjugated tubulin antibody, and phalloidin-conjugated Alexa Fluor 546. Additionally, microsporocytes of L. longiflorum were observed with acetocarmine staining following N2O treatment. A typical metaphase I microtubule distribution was observed in control microsporocytes. After treatment with N2O for 24 h, microtubules were effectively depolymerized; this prevented chromosomes from moving to the poles, resulting in chromosome retention in the center of N2O-treated cells. Cell plate formation took place without delay, however, yielding one daughter cell with a diploid genome and another daughter without chromosomes. In addition, N2O treatment often induced micronuclei due to aberrant chromosome separation during cytokinesis. Actin filaments in microsporocytes are insensitive to N2O. These findings indicate that N2O mediates polyploidization by inhibiting microtubule polymerization, but not actin filament formation, during microsporocyte meiosis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Sexual Plant Reproduction Springer Journals

Mechanism of action of nitrous oxide gas applied as a polyploidizing agent during meiosis in lilies

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Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Life Sciences; Agriculture; Plant Sciences ; Cell Biology
ISSN
2194-7953
eISSN
1432-2145
D.O.I.
10.1007/s00497-008-0084-x
Publisher site
See Article on Publisher Site

Abstract

Nitrous oxide gas (N2O) can be used to produce polyploid plants, but the mechanism of action is unknown. The actin and microtubule cytoskeleton was observed in N2O-treated microsporocytes of Lilium spp ‘Asiatic hybrid lilies’ using fluorescence microscopy after staining with DAPI, FITC-conjugated tubulin antibody, and phalloidin-conjugated Alexa Fluor 546. Additionally, microsporocytes of L. longiflorum were observed with acetocarmine staining following N2O treatment. A typical metaphase I microtubule distribution was observed in control microsporocytes. After treatment with N2O for 24 h, microtubules were effectively depolymerized; this prevented chromosomes from moving to the poles, resulting in chromosome retention in the center of N2O-treated cells. Cell plate formation took place without delay, however, yielding one daughter cell with a diploid genome and another daughter without chromosomes. In addition, N2O treatment often induced micronuclei due to aberrant chromosome separation during cytokinesis. Actin filaments in microsporocytes are insensitive to N2O. These findings indicate that N2O mediates polyploidization by inhibiting microtubule polymerization, but not actin filament formation, during microsporocyte meiosis.

Journal

Sexual Plant ReproductionSpringer Journals

Published: Oct 4, 2008

References

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