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Mapping of the genes for dioecism and monoecism in Spinacia oleracea L.: evidence that both genes are closely linked

Mapping of the genes for dioecism and monoecism in Spinacia oleracea L.: evidence that both genes... Spinach is basically a dioecious species, with occasional monoecious plants in some populations. Sexual dimorphism in dioecious spinach plants is controlled by an allelic pair termed X and Y located on the short arm of the longest chromosome ( x = 6). Ten AFLP markers, closely linked to the X / Y locus, were identified using bulked segregant analysis, four of which were revealed to co-segregate with Y in the present mapping population. We mapped the AFLP markers and two known male-specific DNAs to a 13.4 cM region encompassing the locus. These markers will be the basis for positional cloning of the sex-determination gene. We also showed that a single, incompletely dominant gene is responsible for the highly staminate monoecious character. The gene was found to be located at a distance of 4.3 cM from microsatellite marker SO4, which mapped 1.6 cM from the X / Y locus. This indicates that the monoecious gene seems not to be allelic to but closely linked to the X / Y gene pair. SO4 will enable breeders to efficiently select highly male monoecious plants for preferential use as the pollen parent for hybrid seed production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Mapping of the genes for dioecism and monoecism in Spinacia oleracea L.: evidence that both genes are closely linked

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References (49)

Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Plant Sciences ; Plant Biochemistry; Cell Biology
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-010-0998-2
pmid
21301852
Publisher site
See Article on Publisher Site

Abstract

Spinach is basically a dioecious species, with occasional monoecious plants in some populations. Sexual dimorphism in dioecious spinach plants is controlled by an allelic pair termed X and Y located on the short arm of the longest chromosome ( x = 6). Ten AFLP markers, closely linked to the X / Y locus, were identified using bulked segregant analysis, four of which were revealed to co-segregate with Y in the present mapping population. We mapped the AFLP markers and two known male-specific DNAs to a 13.4 cM region encompassing the locus. These markers will be the basis for positional cloning of the sex-determination gene. We also showed that a single, incompletely dominant gene is responsible for the highly staminate monoecious character. The gene was found to be located at a distance of 4.3 cM from microsatellite marker SO4, which mapped 1.6 cM from the X / Y locus. This indicates that the monoecious gene seems not to be allelic to but closely linked to the X / Y gene pair. SO4 will enable breeders to efficiently select highly male monoecious plants for preferential use as the pollen parent for hybrid seed production.

Journal

Plant Cell ReportsSpringer Journals

Published: Jun 1, 2011

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