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Living cell reaction process for l -isoleucine and l -valine production

Living cell reaction process for l -isoleucine and l -valine production A new process (Living Cell Reaction Process) for l -isoleucine production using viable, non-growing cells of Brevibacterium flavum AB-07 was optimised using ethanol as the energy source and α-ketobutyric acid (α-KB) as precursor. l -valine also could be produced from glucose at high yield by this process. This process differs from the usual fermentation method in that non-growing cells are used, and the production of l -isoleucine and l -valine were carried out under conditions of repressed cell division and growth. Minimal medium missing the essential growth factor, biotin was employed as the reaction mixture for the production of l -isoleucine and l -valine. The productivity of l -isoleucine and l -valine were 200 mmol·l −1 · day −1 (molecular yield to α-KB: 95%) and 300 mmol · l −1 · day −1 (molecular yield to glucose: 80%) respectively. The content of l -isoleucine and l -valine in total amino acids produced in the each mixture were 97% and 96% respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Industrial Microbiology Biotechnology Springer Journals

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References (10)

Publisher
Springer Journals
Copyright
Copyright © 1990 by Society for Industrial Microbiology
Subject
Chemistry; Biotechnology; Genetic Engineering; Biochemistry, general; Bioinformatics; Microbiology; Microbial Genetics and Genomics
ISSN
1367-5435
eISSN
1476-5535
DOI
10.1007/BF01578203
Publisher site
See Article on Publisher Site

Abstract

A new process (Living Cell Reaction Process) for l -isoleucine production using viable, non-growing cells of Brevibacterium flavum AB-07 was optimised using ethanol as the energy source and α-ketobutyric acid (α-KB) as precursor. l -valine also could be produced from glucose at high yield by this process. This process differs from the usual fermentation method in that non-growing cells are used, and the production of l -isoleucine and l -valine were carried out under conditions of repressed cell division and growth. Minimal medium missing the essential growth factor, biotin was employed as the reaction mixture for the production of l -isoleucine and l -valine. The productivity of l -isoleucine and l -valine were 200 mmol·l −1 · day −1 (molecular yield to α-KB: 95%) and 300 mmol · l −1 · day −1 (molecular yield to glucose: 80%) respectively. The content of l -isoleucine and l -valine in total amino acids produced in the each mixture were 97% and 96% respectively.

Journal

Journal of Industrial Microbiology BiotechnologySpringer Journals

Published: Jul 1, 1990

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