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Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis

Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis Glycosylation is one of the most significant and abundant post-translational modifications in cells. Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycomic and glycoproteomic analysis is highly challenging because of the large diversity of structures, low abundance, site-specific heterogeneity, and poor ionization efficiency of glycans and glycopeptides in mass spectrometry (MS). MS is a key tool for characterization of glycans and glycopeptides. However, MS alone does not always provide full structural and quantitative information for many reasons, and thus MS is combined with some separation technique. This review focuses on the role of separation techniques used in glycomic and glycoproteomic analyses, liquid chromatography and capillary electrophoresis. The most important separation conditions and results are presented and discussed.Graphical abstract[graphic not available: see fulltext] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Monatshefte für Chemie - Chemical Monthly Springer Journals

Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis

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References (186)

Publisher
Springer Journals
Copyright
Copyright © Springer-Verlag GmbH Austria, part of Springer Nature 2022
ISSN
0026-9247
eISSN
1434-4475
DOI
10.1007/s00706-022-02938-4
Publisher site
See Article on Publisher Site

Abstract

Glycosylation is one of the most significant and abundant post-translational modifications in cells. Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycomic and glycoproteomic analysis is highly challenging because of the large diversity of structures, low abundance, site-specific heterogeneity, and poor ionization efficiency of glycans and glycopeptides in mass spectrometry (MS). MS is a key tool for characterization of glycans and glycopeptides. However, MS alone does not always provide full structural and quantitative information for many reasons, and thus MS is combined with some separation technique. This review focuses on the role of separation techniques used in glycomic and glycoproteomic analyses, liquid chromatography and capillary electrophoresis. The most important separation conditions and results are presented and discussed.Graphical abstract[graphic not available: see fulltext]

Journal

Monatshefte für Chemie - Chemical MonthlySpringer Journals

Published: Sep 1, 2022

Keywords: Capillary zone electrophoresis; Glycan separation; Glycopeptide separation; High-pressure liquid chromatography; Proteomics

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