Access the full text.
Sign up today, get DeepDyve free for 14 days.
(2000)
IB Market Forecast
D. Friedberg, Y. Peleg, A. Monsonego, S. Maissi, E. Battat, J. Rokem, I. Goldberg (1995)
The fumR gene encoding fumarase in the filamentous fungus Rhizopus oryzae: cloning, structure and expression.Gene, 163 1
K. Watkins (2002)
A SOLVENT BUSINESSChemical & Engineering News, 80
C. Skory (2003)
Lactic acid production by Saccharomyces cerevisiae expressing a Rhizopus oryzae lactate dehydrogenase geneJournal of Industrial Microbiology and Biotechnology, 30
S. Dequin, P. Barré (1994)
Mixed Lactic Acid–Alcoholic Fermentation by Saccharomyes cerevisiae Expressing the Lactobacillus casei L(+)–LDHBio/Technology, 12
Barbara Bakker, K. Overkamp, van AJ, P. Kötter, M. Luttik, van JP, J. Pronk (2001)
Stoichiometry and compartmentation of NADH metabolism in Saccharomyces cerevisiae.FEMS microbiology reviews, 25 1
손응룡, 박원목, 이용세, 안상득, 천성룡 (1984)
人蔘品種間 Isozyme pattern 比較, 29
D. Porro, M. Bianchi, L. Brambilla, R. Menghini, Davide Bolzani, Vittorio Carrera, J. Lievense, Chi-Li Liu, B. Ranzi, L. Frontali, L. Alberghina (1999)
Replacement of a Metabolic Pathway for Large-Scale Production of Lactic Acid from Engineered YeastsApplied and Environmental Microbiology, 65
M. Taherzadeh, L. Adler, G. Lidén (2002)
Strategies for enhancing fermentative production of glycerol—a reviewEnzyme and Microbial Technology, 31
Y. Peleg, E. Battat, M. Scrutton, I. Goldberg (1989)
Isoenzyme pattern and subcellular localisation of enzymes involved in fumaric acid accumulation by Rhizopus oryzaeApplied Microbiology and Biotechnology, 32
P. Bunch, F. Mat-Jan, N. Lee, D. Clark (1997)
The IdhA Gene Encoding the Fermentative Lactate Dehydrogenase of Escherichia ColiMicrobiology, 143
K. Watkins (2002)
Sustainability takes center stageChemical & Engineering News, 80
Stephen Osmani, M. Scrutton (1985)
The sub-cellular localisation and regulatory properties of pyruvate carboxylase from Rhizopus arrhizus.European journal of biochemistry, 147 1
(2001)
Fungal lactate dehydrogenase gene and constructs for the expression thereof
C. Skory (2003)
Induction of Rhizopus oryzae Pyruvate Decarboxylase GenesCurrent Microbiology, 47
W. Kenealy, E. Zaady, J. Preez, B. Stieglitz, I. Goldberg (1986)
Biochemical Aspects of Fumaric Acid Accumulation by Rhizopus arrhizusApplied and Environmental Microbiology, 52
C. Skory (2002)
Homologous recombination and double-strand break repair in the transformation of Rhizopus oryzaeMolecular Genetics and Genomics, 268
C. Skory (2000)
Isolation and Expression of Lactate Dehydrogenase Genes from Rhizopus oryzaeApplied and Environmental Microbiology, 66
E. Adachi, Mikiko Torigoe, M. Sugiyama, J. Nikawa, K. Shimizu (1998)
Modification of metabolic pathways of Saccharomyces cerevisiae by the expression of lactate dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH valueJournal of Fermentation and Bioengineering, 86
Rhizopus oryzae is capable of producing high levels of lactic acid by the fermentation of glucose. Yields typically vary over 60–80%, with the remaining glucose diverted primarily into ethanol fermentation. The goal of this work was to increase lactate dehydrogenase (LDH) activity, so lactic acid fermentation could more effectively compete for available pyruvate. Three different constructs, pLdhA71X, pLdhA48XI, and pLdhA89VII, containing various lengths of the ldhA gene fragment, were transformed into R. oryzae. This fungus rarely integrates DNA used for transformation, but instead relies on extra-chromosomal replication in a high-copy number. Plasmid pLdhA48XI was linearized prior to transformation in order to facilitate integration into the pyrG gene used for selection. Isolates transformed with ldhA containing plasmid were compared with both the wild-type parent strain and the auxotrophic recipient strain containing vector only. All isolates transformed with pLdhA71X or pLdhA48XI had multiple copies of the ldhA gene that resulted in ldhA transcript accumulation, LDH specific activity, and lactic acid production higher than the controls. Integration of plasmid pLdhA48XI increased the stability of the strain, but did not seem to offer any benefit for increasing lactic acid production. Since lactic acid fermentation competes with ethanol and fumaric acid production, it was not unexpected that increased lactic acid production was always concomitant with decreased ethanol and fumaric acid. Plasmid pLdhA71X, containing a large ldhA fragment (6.1 kb), routinely yielded higher levels of lactic acid than the smaller region (3.3 kb) used to construct plasmid pLdhA48XI. The greatest levels of ldhA transcript and enzyme production occurred with isolates transformed with plasmid pLdhA89VII. However, these transformants always produced less lactic acid and higher amounts of ethanol, fumaric, and glycerol compared with the control.
Applied Microbiology and Biotechnology – Springer Journals
Published: Nov 18, 2003
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.