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Labeling of oligonucleotides with fluorescein

Labeling of oligonucleotides with fluorescein The labeling of oligonucleotide probes using a fluorescein-labeled nucleotide is described. The reaction is characterized by careful control of the nucleotide and probe molar ratio in order to produce a tail that gives good detection sensitivity without compromising hybridization stringency control of the probe sequence. The labeling reaction can be easily monitored for incorporation of the fluorescent label and the probes can be used in many applications. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Biotechnology Springer Journals

Labeling of oligonucleotides with fluorescein

Chadwick, Patricia; Durrant, Ian
Molecular Biotechnology , Volume 7 (3) – Nov 3, 2007
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References (6)

Publisher
Springer Journals
Copyright
Copyright © 1997 by Humana Press Inc
Subject
Biomedicine; Neurosciences; Neurology
ISSN
1073-6085
eISSN
1559-0305
DOI
10.1007/BF02740820
pmid
9219243
Publisher site
See Article on Publisher Site

Abstract

The labeling of oligonucleotide probes using a fluorescein-labeled nucleotide is described. The reaction is characterized by careful control of the nucleotide and probe molar ratio in order to produce a tail that gives good detection sensitivity without compromising hybridization stringency control of the probe sequence. The labeling reaction can be easily monitored for incorporation of the fluorescent label and the probes can be used in many applications.

Journal

Molecular Biotechnology – Springer Journals

Published: Nov 3, 2007

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