Knockout rats generated by embryo microinjection of TALENs

Knockout rats generated by embryo microinjection of TALENs To the Editor: The recent description of highly active transcription activator-like effector nucleases (TALENs) prompted us to explore their utility for in vivo genetic engineering in the laboratory rat. The rat is a valuable experimental animal because of its suitability for modeling human disease and toxicology. Zinc-finger nuclease (ZFN) technology and the isolation of rat embryonic stem cells have enabled targeted modifications of the rat genome . Recently, Xanthomonas -derived transcription activator-like (TAL) effector proteins have elicited much interest because of their apparently simple rules for sequence-specific DNA recognition . Several investigators have fused the FokI nuclease domain to TAL effector proteins to create TALENs . However, only optimal truncation of the TAL effector protein allowed high-frequency gene disruption of endogenous loci and targeted DNA integration . Here we use TALENs to disrupt the rat IgM locus, creating heritable mutations that eliminate IgM function. Our results establish the use of TALEN technology for in vivo gene knockout in mammals. We designed and assembled TALENs to exon 2 of rat IgM and tested their ability to alter the IgM locus in rat S16 cells ( Fig. 1a and Supplementary Sequences ). The TALEN pair modified ∼21% of chromosomes when http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Biotechnology Springer Journals

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Publisher
Springer Journals
Copyright
Copyright © 2011 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
ISSN
1087-0156
eISSN
1546-1696
DOI
10.1038/nbt.1940
Publisher site
See Article on Publisher Site

Abstract

To the Editor: The recent description of highly active transcription activator-like effector nucleases (TALENs) prompted us to explore their utility for in vivo genetic engineering in the laboratory rat. The rat is a valuable experimental animal because of its suitability for modeling human disease and toxicology. Zinc-finger nuclease (ZFN) technology and the isolation of rat embryonic stem cells have enabled targeted modifications of the rat genome . Recently, Xanthomonas -derived transcription activator-like (TAL) effector proteins have elicited much interest because of their apparently simple rules for sequence-specific DNA recognition . Several investigators have fused the FokI nuclease domain to TAL effector proteins to create TALENs . However, only optimal truncation of the TAL effector protein allowed high-frequency gene disruption of endogenous loci and targeted DNA integration . Here we use TALENs to disrupt the rat IgM locus, creating heritable mutations that eliminate IgM function. Our results establish the use of TALEN technology for in vivo gene knockout in mammals. We designed and assembled TALENs to exon 2 of rat IgM and tested their ability to alter the IgM locus in rat S16 cells ( Fig. 1a and Supplementary Sequences ). The TALEN pair modified ∼21% of chromosomes when

Journal

Nature BiotechnologySpringer Journals

Published: Aug 5, 2011

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