Karyotype, genome size, and in vitro chromosome doubling of Pfaffia glomerata (Spreng.) Pedersen

Karyotype, genome size, and in vitro chromosome doubling of Pfaffia glomerata (Spreng.) Pedersen Pfaffia glomerata (Spreng.) Pedersen, known worldwide as Brazilian ginseng, has an important commercial value due to its pharmaceutical properties. In addition to the newly described karyological traits and the first estimation of DNA content, this study reports a protocol for the successful induction of tetraploidy. Natural diploid individuals (2n = 34) showed a symmetric karyotype, centromeric DAPI+ bands, one chromosome pair with a CMA+ band and 45S rDNA site and another with one 5S rDNA site. To induce chromosome duplication, small nodal buds were cultured in semi-solid MS-based medium with 2.22 μM BA, 2.69 μM NAA, and colchicine or oryzalin at 10, 15, 20, 25, and 30 μM for 1 or 2 weeks before being transferred to MS basal medium. The results showed that colchicine induced tetraploid plants, mainly after 1 week of exposure, whereas oryzalin treatment induced only mixoploid plants. The tetraploid plants exhibited twice the chromosome number and DNA content and twice the number of chromosome markers observed for the diploids. Chromosome duplication reduced the dry mass of the stems and roots of the polyploid plants compared to the diploids, and the stomatal density was also reduced on the abaxial and adaxial leaf surfaces of the polyploids. Additionally, the production of β-ecdysone was 50 % higher in the tetraploids than in the diploids. Thus, chromosome doubling showed that is possible to increase the content of β-ecdysone, highlighting the considerable potential of this technique to produce new cultivars with high commercial value. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell, Tissue and Organ Culture Springer Journals

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Plant Physiology; Plant Genetics & Genomics; Plant Pathology
ISSN
0167-6857
eISSN
1573-5044
D.O.I.
10.1007/s11240-014-0460-1
Publisher site
See Article on Publisher Site

Abstract

Pfaffia glomerata (Spreng.) Pedersen, known worldwide as Brazilian ginseng, has an important commercial value due to its pharmaceutical properties. In addition to the newly described karyological traits and the first estimation of DNA content, this study reports a protocol for the successful induction of tetraploidy. Natural diploid individuals (2n = 34) showed a symmetric karyotype, centromeric DAPI+ bands, one chromosome pair with a CMA+ band and 45S rDNA site and another with one 5S rDNA site. To induce chromosome duplication, small nodal buds were cultured in semi-solid MS-based medium with 2.22 μM BA, 2.69 μM NAA, and colchicine or oryzalin at 10, 15, 20, 25, and 30 μM for 1 or 2 weeks before being transferred to MS basal medium. The results showed that colchicine induced tetraploid plants, mainly after 1 week of exposure, whereas oryzalin treatment induced only mixoploid plants. The tetraploid plants exhibited twice the chromosome number and DNA content and twice the number of chromosome markers observed for the diploids. Chromosome duplication reduced the dry mass of the stems and roots of the polyploid plants compared to the diploids, and the stomatal density was also reduced on the abaxial and adaxial leaf surfaces of the polyploids. Additionally, the production of β-ecdysone was 50 % higher in the tetraploids than in the diploids. Thus, chromosome doubling showed that is possible to increase the content of β-ecdysone, highlighting the considerable potential of this technique to produce new cultivars with high commercial value.

Journal

Plant Cell, Tissue and Organ CultureSpringer Journals

Published: Mar 14, 2014

References

  • Morphological, physiological, cytological and phytochemical studies in diploid and colchicine-induced tetraploid plants of Echinacea purpurea (L.)
    Abdoli, M; Moieni, A; Badi, HN
  • In vitro induction of tetraploid ginger (Zingiber officinale Roscoe) and its pollen fertility and germinability
    Adaniya, S; Shira, D
  • In vitro induction of tetraploids in Arachis paraguariensis
    Aina, O; Quesenberry, K; Gallo, M
  • Transcriptomic shock generates evolutionary novelty in a newly formed, natural allopolyploid plant
    Buggs, RJA; Zhang, LJ; Miles, N; Tate, JA; Gao, L; Wei, W; Schnable, PS; Barbazuk, WB; Soltis, PS; Soltis, DE
  • Rapid, repeated, and clustered loss of duplicate genes in allopolyploid plant populations of independent origin
    Buggs, RJA; Chamala, S; Wu, W; Tate, JA; Schnable, PS; Soltis, PS; Soltis, DE; Barbazuk, WB
  • In vitro tetraploid induction and generation of tetraploids from mixoploids in Astragalus membranaceus
    Chen, LL; Gao, SL
  • Enhancing the productivity of secondary metabolites via induced polyploidy: a review
    Dhawan, OP; Lavania, UC

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