Isolation of the Promoters of Atlantic Salmon MHCII Genes

Isolation of the Promoters of Atlantic Salmon MHCII Genes The major histocompatibility complex class II (MHCII) has a central role in the immune response of vertebrates with its function of presenting antigenic peptides to the T-cell receptors. We have isolated the promoters and intron 1 of MHCIIα and MHCIIβ genes of Atlantic salmon. To isolate these promoters, we constructed an Atlantic salmon (Salmo salar) promoter finder kit (analogous to the commercially available “human promoter finder kit”). By nucleotide sequence alignment of known MHCII promoter regions, we identified the 3 conserved regulatory X, X2, and Y boxes in the salmon promoters. The W box was not found. In contrast, a salmon-specific putative W box was identified. Both of the isolated Atlantic salmon MHCIIα and β promoters (included in patent applications by Genomar A/S, Oslo, Norway) were found to be functional since they both gave positive yellow fluorescence protein signal when inserted as promoters in the pEYFP-1 reporter plasmid and transfected into the salmon head kidney cell line (SHK-1). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Marine Biotechnology Springer Journals

Isolation of the Promoters of Atlantic Salmon MHCII Genes

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Publisher
Springer Journals
Copyright
Copyright © 2003 by Springer-Verlag New York Inc.
Subject
Philosophy
ISSN
1436-2228
D.O.I.
10.1007/s10126-002-0063-4
Publisher site
See Article on Publisher Site

Abstract

The major histocompatibility complex class II (MHCII) has a central role in the immune response of vertebrates with its function of presenting antigenic peptides to the T-cell receptors. We have isolated the promoters and intron 1 of MHCIIα and MHCIIβ genes of Atlantic salmon. To isolate these promoters, we constructed an Atlantic salmon (Salmo salar) promoter finder kit (analogous to the commercially available “human promoter finder kit”). By nucleotide sequence alignment of known MHCII promoter regions, we identified the 3 conserved regulatory X, X2, and Y boxes in the salmon promoters. The W box was not found. In contrast, a salmon-specific putative W box was identified. Both of the isolated Atlantic salmon MHCIIα and β promoters (included in patent applications by Genomar A/S, Oslo, Norway) were found to be functional since they both gave positive yellow fluorescence protein signal when inserted as promoters in the pEYFP-1 reporter plasmid and transfected into the salmon head kidney cell line (SHK-1).

Journal

Marine BiotechnologySpringer Journals

Published: Sep 19, 2003

References

  • Map of the human MHC.
    Cambell, R.D.; Trowsdale, J.
  • Polymorphic MHC class II promoters exhibit distinct expression pattern in various antigen-presenting cell lines.
    Janitz, M.; Mitchison, A.; Reiners-Schramm, L.; Lauster, R.

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