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Investigation of the influence of NaCl concentration on Halobacterium salinarum growth

Investigation of the influence of NaCl concentration on Halobacterium salinarum growth Microcalorimetry was used to study the influence of NaCl concentration on Halobacterium salinarum growth. From the thermogenic curves and thermokinetic parameters of H. salinarum growth in different concentrations of NaCl, it was found that the optimum NaCl concentration for H. salinarum growth was not a wide range from 3.5 mol L –1 to NaCl saturation (about 5.2 mol L –1 ), as is generally acknowledged, but just around 230 g L –1 (approximately 3.9 mol L –1 ). And when external NaCl concentration was above 230 g L –1 , the growth metabolism of H. salinarum decreased constantly with the increasing of NaCl concentration. These have never been described before. Further investigation by transmission electron microscopy revealed that H. salinarum growing in approaching NaCl saturation underwent plasmolysis, which interpreted the novel finding of microcalorimetry perfectly. Our work shows that microcalorimetry may reveal more and newer details about microbial growth than the existing methods do. These details are significant to understand biological processes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Thermal Analysis and Calorimetry Springer Journals

Investigation of the influence of NaCl concentration on Halobacterium salinarum growth

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References (13)

Publisher
Springer Journals
Copyright
Copyright © 2006 by Springer-Verlag
Subject
Chemistry; Inorganic Chemistry; Physical Chemistry; Polymer Sciences; Measurement Science, Instrumentation
ISSN
1388-6150
eISSN
1572-8943
DOI
10.1007/s10973-005-9988-y
Publisher site
See Article on Publisher Site

Abstract

Microcalorimetry was used to study the influence of NaCl concentration on Halobacterium salinarum growth. From the thermogenic curves and thermokinetic parameters of H. salinarum growth in different concentrations of NaCl, it was found that the optimum NaCl concentration for H. salinarum growth was not a wide range from 3.5 mol L –1 to NaCl saturation (about 5.2 mol L –1 ), as is generally acknowledged, but just around 230 g L –1 (approximately 3.9 mol L –1 ). And when external NaCl concentration was above 230 g L –1 , the growth metabolism of H. salinarum decreased constantly with the increasing of NaCl concentration. These have never been described before. Further investigation by transmission electron microscopy revealed that H. salinarum growing in approaching NaCl saturation underwent plasmolysis, which interpreted the novel finding of microcalorimetry perfectly. Our work shows that microcalorimetry may reveal more and newer details about microbial growth than the existing methods do. These details are significant to understand biological processes.

Journal

Journal of Thermal Analysis and Calorimetry – Springer Journals

Published: May 1, 2006

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