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ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 8, pp. 788–796. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2018, published in Biotekhnologiya, 2018, Vol. 34, No. 6, pp. 33–42. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Increase in the Thermal Stability of Phytase from Citrobacter freundii by Site-Directed Saturation Mutagenesis a, a a a T. L. Gordeeva *, L. N. Borshchevskaya , A. N. Kalinina , S. P. Sineoky , b b M. D. Kashirskaya , and S. P. Voronin State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GOSNIIGENETIKA, Kurchatov Institute NRC), Moscow, 117545 Russia BIOAMIDE Joint-Stock Venture, Saratov, 410033 Russia *e-mail: tatiana.gordeeva@mail.ru Received October 12, 2018; revised November 13, 2018; accepted December 15, 2018 Abstract—Phytases are widely used to improve the properties of mixed fodder. Due to its high specif ic activity, PhyА-Cf phytase from Citrobacter freundii is of special interest for agrobiotechnology. In order to decrease the thermal sensitivity of the enzyme during fodder granulation at 60–80°C, the thermal stability of phytase was enhanced by site-directed saturation mutagenesis. The mutant genes were cloned in a pP10 vector under the control of the GAP promoter and expressed
Applied Biochemistry and Microbiology – Springer Journals
Published: Dec 5, 2019
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