Plant Mol Biol (2016) 92:357–369 DOI 10.1007/s1103-016-0518-z1 In vitro pr romoter ecognition by the catalytic subunit of plant phage-type RNA polymerases 1,2 1 1,3 1 iolaAlexandra-V Bohne · Marlene eubnerT · Karsten eLier · Andreas eiheW · Thomas Börner Received: 28 January 2016 / Accepted: 20 July 2016 / Published online: 6 August 2016 © The Author(s) 2016. This article is published with open access at Springerlink.com Abstract ferent dif truncated and mutagenized promoter constructs, we Key message We identified sequence motifs, which observed the same minimal promoter sequence supposed to enhance or reduce the ability of the Arabidopsis phage- be needed in vivo for transcription initiation. , we Moreover type RNA polymerases RPOTm (mitochondrial RNAP), identified elements of core and flanking sequences, which RPOTp (plastidial RNAP), and RPOTmp (active in both are of critical importance for promoter recognition and organelles) to recognize prtheir omoters in vitro with activity in vitro. e further intended W to reveal why RPOTmp help of a ‘specificity loop’. The importance of this data does not efficiently recognize promoters in vitro and if pro - the evolution and for function of the organellar RNA moter recognition is based on a structurally defined speci -
Plant Molecular Biology – Springer Journals
Published: Aug 6, 2016
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