Access the full text.
Sign up today, get DeepDyve free for 14 days.
G. Acker (1988)
7 Immunoelectron Microscopy of Surface Antigens (Polysaccharides) of Gram-negative Bacteria using Pre- and Post-embedding Techniques*Methods in Microbiology, 20
(1987)
~dber Sukzession und pathogene
(1985)
Use of monoclonal antibodies to detect a phytotoxic glycolipide
(1981)
Infection of Pinus sylvestris by Naemacyclus minor
(1980)
CMI-Description of pathogenic fungi and bacteria, set
N. Benhamou, G. Ouellette, J. Lafontaine, J. Joly (1985)
Use of monoclonal antibodies to detect a phytotoxic glycopeptide produced by Ophiostoma ulmi, the Dutch elm disease pathogenBotany, 63
B. Walles, B. Nyman, T. Aldén (1973)
On the ultrastructure of needles of Pinus silvestris L.
R. Blanchette, A. Abad (1992)
Immunocytochemistry of Fungal Infection Processes in Trees
D. Hinton, C. Bacon (1985)
The distribution and ultrastructure of the endophyte of toxic tall fescueBotany, 63
S. Gianinazzi, V. Gianinazzi‐Pearson (1992)
Cytology, Histochemistry and Immunocytochemistry as Tools for Studying Structure and Function in EndomycorrhizaMethods in Microbiology, 24
G. Darker (1932)
The Hypodermataceae of conifers., 1
J. Suske, G. Acker (1989)
Identification of endophytic hyphae of Lophodermium piceae in tissues of green, symptomless Norway spruce needles by immunoelectron microscopyBotany, 67
(1989)
Krankheiten der Wald- und Parkb/iume, 2. Aufl
J. Arx (1975)
The genera of fungi sporulating in pure culture
E. Reynolds (1963)
THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPYThe Journal of Cell Biology, 17
N. Benhamou, G. Ouellette (1987)
Ultrastructural study and cytochemical investigation, by means of enzyme–gold complexes, of the fungus Ascocalyx abietinaBotany, 65
K. Rack, U. Scheidemann (1987)
Über Sukzession und pathogene Eigenschaften Kiefernnadeln bewohnender PilzeForest Pathology, 17
M. Ellis (1976)
More Dematiaceous Hyphomycetes
A. Spurr (1969)
A low-viscosity epoxy resin embedding medium for electron microscopy.Journal of ultrastructure research, 26 1
S. Perotto, F. Malavasi, G. Butcher (1992)
Use of monoclonal antibodies to study mycorrhiza: Present applications and perspectivesMethods in Microbiology, 24
L. Lim, B. Fineran, A. Cole (1983)
ULTRASTRUCTURE OF INTRAHYPHAL HYPHAE OF GLOMUS FASCICULA TUM (THAXTER) GERDEMANN AND TRAPPE IN ROOTS OF WHITE CLOVER (TRIFOLIUM REPENS L.)New Phytologist, 95
P. Gadgil (1984)
Cyclaneusma (Naemacyclus) needle-cast of Pinus radiata in New Zealand. 1: Biology of Cyclaneusma minus.New Zealand journal of forestry science, 14
(1982)
The protein A-gold (pAg) technique - a qualitaUve
C. Bracker (1973)
III.3 – Structural Concepts of Host–Pathogen Interfaces*
J. Pendland (1982)
Resistant structures in the entomogenous hyphomycete, Nomuraea rileyi: an ultrastructural studyBotany, 60
(1991)
Extracellular materials of fungai structures : their significance at penetration stages 9 of infection
C. Booth, P. Kirk, D. Hawksworth, J. Mordue, B. Brady (1979)
CMI Descriptions of Pathogenic Fungi and Bacteria, 601610
M. Bernstein, G. Carroll (1977)
Internal fungi in old-growth Douglas fir foliageBotany, 55
(1984)
Experimenteller Nachweis nadelbe
S. Huber, B. Hock (1985)
A Solid-phase enzyme immunoassay for quantitative determination of the herbicide terbutryn, 92
J. Suske, G. Acker (1989)
Endophytic Needle Fungi: Culture, Ultrastructural and Immunocytochemical Studies
(1992)
Use of monoclonal
(1973)
Structural concepts of hostpathogen interfaces
H. Kunoh, R. Nicholson, I. Kobayashi (1991)
Extracellular Materials of Fungal Structures: Their Significance at Prepenetration Stages of Infection
(1973)
On the ultrastrueture of Pinus sylvestris L
I. Gibson (1975)
Diseases of forest trees widely planted as exotics in the Tropics and Southern Hemisphere
G. Darker (1967)
A REVISION OF THE GENERA OF THE HYPODERMATACEAEBotany, 45
K. Rack, H. Butin (1984)
Experimenteller Nachweis nadelbewohnender Pilze bei Koniferen. I. Fichte (Picea abies)Forest Pathology, 14
(1989)
Identification of endophytic hyphae
T. Kowalski (1988)
Cyclaneusma (Naemacyclus) minus an Pinus sylvestris in PolenForest Pathology, 18
B. Kistler (1978)
Etiology, Symptomology, Epidemiology, and Control of Naemacyclus Needlecast of Scotch PinePhytopathology, 68
T. Kowalski (1982)
Fungi infecting Pinus sylvestris needles of various agesForest Pathology, 12
J. Webster, M. Ellis, J. Ellis (1986)
Microfungi on Land Plants.Journal of Ecology, 74
M. Ellis, J. Ellis (1985)
Microfungi on Land Plants: An Identification Handbook
G. Darker (1967)
A REVISION OF THE GENERA OF THE HYPODERMATACEAEl
T. Kowalski, K. Lang (1983)
Über die Mykoflora in den Nadeln unterschiedlich alter Kiefern (Pinus sylvestris L.)Journal of Phytopathology, 107
G. Acker, W. Knapp, K. Wartenberg, H. Mayer (1981)
Localization of enterobacterial common antigen in Yersinia enterocolitica by the immunoferritin techniqueJournal of Bacteriology, 147
J. Suske, G. Acker (1990)
Host-endophyte interaction between Lophodermium piceae and Picea abies: cultural, ultrastructural and immunocytochemical studies.Sydowia, 42
203 160 160 4 4 Felix Franz Rita Grotjahn Georg Acker Fachgruppe Biologie/Elektronenmikroskopie Universität Bayreuth Postfach 10 12 51 D-95440 Bayreuth Germany Abstract Cultural investigations revealed that Naemacyclus minor, Lophodermium seditiosum and Cenangium ferruginosum were the most frequent colonizers of asymptomatic and symptomatic Pinus sylvestris needles. Since ultrastructural observations showed that morphological features were not suitable to differentiate hyphae of N. minor from hyphae of other isolates, the on-section immunogold labelling technique was applied in combination with an anti- N. minor specific immunoserum. The specificity of this serum was tested against culture hyphae of all isolates. Anti- N. minor specific immunoserum was then used to identify N. minor hyphae in thin sections of green P. sylvestris needles. The infection loci identified were restricted to small tissue areas located in the vicinity of stomata. In the hypodermis, hyphae and endocell-containing hyphae were located within the lumina of host cells but outside the protoplast. The growth of hyphae from cell to cell occurred through pits. The hyphae spreat into the mesophyll intercellularly and continued with the intracellular colonization of moribund and dead mesophyll cells in a later stage of infection. The observed host-parasite interactions at cellular and ultrastructural level are discussed in connection with the still controversial interpretation of the pathogenicity of N. minor .
Archives of Microbiology – Springer Journals
Published: Oct 1, 1993
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.