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- Publisher
- Springer Journals
- Copyright
- Copyright © 2010 by Springer Science+Business Media, LLC
- Subject
- Chemistry; Biochemistry, general; Biotechnology
- ISSN
- 0273-2289
- eISSN
- 1559-0291
- DOI
- 10.1007/s12010-010-9111-0
- pmid
- 20972891
- Publisher site
- See Article on Publisher Site
Abstract
To improve the enzymatic hydrolytic efficiency and reduce the supplementation of enzymes, the mixture designed experimental approach was used to optimize the composition of enzyme mixture and promote the hydrolysis of ball-milled corn stover. From the experimental results, a synergistic effect was found when combinations of the three enzymes, two kinds of cellulases and a kind of xylanase, were used. The optimal hydrolysis of pretreated corn stover accorded with enzymes activity ration of FPU/CMCase/β-glucosidase/xylanase = 4.4:1:75:829, and the hydrolysis efficiency of corn stover increased significantly compared with using individual enzyme. The results indicated that the mixture design experiment could be an effective tool for optimized enzyme mixture for lignocellulose hydrolysis.
Journal
Applied Biochemistry and Biotechnology – Springer Journals
Published: Oct 24, 2010