Histone-like protein H-NS as a negative regulator of quorum sensing systems in gram-negative bacteria

Histone-like protein H-NS as a negative regulator of quorum sensing systems in gram-negative... The effects of histone-like protein H-NS on transcription of promoters of the Quorum Sensing regulated operons from marine luminescent mesophilic bacterium Aliivibrio fischeri and psychrophilic Aliivibrio logei, as well as from pathogenic Pseudomonas aeruginosa, are studied. In the present work, the plasmids carrying DNA fragments with the promoters Pr1f (upstream of the luxICDABEG operon from A. fischeri), Pr1l (upstream of the luxCDABEG operon from A. logei), Pr2l (upstream of luxI gene from A. logei), PluxCf (upstream of luxC gene from A. fischeri), and PlasI (upstream of lasI gene from P. aerugenosa) are used. In these plasmids, promoter-operator regions are transcriptionally fused to the reporter genes cassette luxCDABE from Photorhabdus luminescens. Here we have shown that the transcription of the QS-regulated promoters in E. coli hns::kan cells increases 100 to 1000 times. Furthermore, transcription of the QS-regulated promoters in E. coli hns + cells increases 10 to 100 times in the cells transformed with the plasmid carrying gene ardA ColIb-P9 encoding DNA mimic antirestriction protein ArdA, inhibitor of the type I restriction-modification systems. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Histone-like protein H-NS as a negative regulator of quorum sensing systems in gram-negative bacteria

8 pages
Read Article

Loading next page...
 
/lp/springer-journals/histone-like-protein-h-ns-as-a-negative-regulator-of-quorum-sensing-sRNLDSIvoH
Publisher site
See Article on Publisher Site

Abstract

The effects of histone-like protein H-NS on transcription of promoters of the Quorum Sensing regulated operons from marine luminescent mesophilic bacterium Aliivibrio fischeri and psychrophilic Aliivibrio logei, as well as from pathogenic Pseudomonas aeruginosa, are studied. In the present work, the plasmids carrying DNA fragments with the promoters Pr1f (upstream of the luxICDABEG operon from A. fischeri), Pr1l (upstream of the luxCDABEG operon from A. logei), Pr2l (upstream of luxI gene from A. logei), PluxCf (upstream of luxC gene from A. fischeri), and PlasI (upstream of lasI gene from P. aerugenosa) are used. In these plasmids, promoter-operator regions are transcriptionally fused to the reporter genes cassette luxCDABE from Photorhabdus luminescens. Here we have shown that the transcription of the QS-regulated promoters in E. coli hns::kan cells increases 100 to 1000 times. Furthermore, transcription of the QS-regulated promoters in E. coli hns + cells increases 10 to 100 times in the cells transformed with the plasmid carrying gene ardA ColIb-P9 encoding DNA mimic antirestriction protein ArdA, inhibitor of the type I restriction-modification systems.

Journal

Russian Journal of Genetics – Springer Journals

Published: Mar 11, 2017

References

  • Quorum Sensing: cell-tocell communication in bacteria
    Waters, C.; Bassler, B.
  • Quorum sensing regulation of gene expression: a promising target for drugs against bacterial pathogenicity
    Khmel, I.A.; Metlitskaya, A.Z.
  • Quorum sensing: how bacteria can coordinate activity and synchronize their response to external signals?
    Li, Z.; Nair, S.K.
  • Quorum sensing in bacteria: the LuxR–LuxI family of cell density–responsive transcriptional regulators
    Fuqua, W.C.; Winans, S.C.; Greenberg, E.P.
  • Census and consensus in bacterial ecosystems: the LuxR–LuxI family of quorum sensing transcriptional regulators
    Fuqua, W.C.; Winans, S.C.; Greenberg, E.P.
  • Physiological
    Meighen, E.A.; Dunlap, P.V.
  • H-NS protein represses transcription of the lux systems of Vibrio fischeri and other luminous bacteria cloned into Escherichia coli
    Ulitzur, S.; Matin, A.; Fraley, C.; Meighen, E.
  • Molecular analysis of the Escherichia coli hns gene encoding a DNA-binding protein, which preferentially recognizes curved DNA sequences
    Yamada, H.; Yoshida, T.; Tanaka, K.
  • The chromatin-associated protein H-NS interacts with curved DNA to influence DNA topology and gene expression
    Owen-Hughes, T.A.; Pavitt, G.D.; Santos, D.S.
  • Twelve species of the nucleoid-associated protein from Escherichia coli: sequence recognition specificity and DNA binding affinity
    Azam, T.A.; Ishichama, A.
  • The Escherichia coli nucleoid protein H-NS functions directly as a transcriptional repressor
    Ueguchi, C.; Mizuno, T.
  • H-NS: a modulator of environmentally regulated gene expression
    Atlung, T.; Ingmer, H.
  • H-NS promotes looped domain formation in the bacterial chromosome
    Noom, M.C.; Navarre, W.W.; Oshima, T.
  • H-NS cooperative binding to high-affinity sites in a regulatory element results in transcriptional silencing
    Bouffartigues, E.; Buckle, M.; Badaut, C.
  • H-NS, the genome sentinel
    Dorman, C.J.
  • Silencing of foreign DNA in bacteria
    Ali, S.S.; Xia, B.; Liu, J.; Navarre, W.W.
  • H-NS-like nucleotid-associated proteins, mobile genetic elements and horizontal gene transfer in bacteria
    Dorman, C.J.
  • The quorum-sensing transcriptional regulator TraR requires its cognate signaling ligand for protein folding
    Zhu, J.; Winans, S.C.
  • Comparative analysis of the lux operons in Aliivibrio logei Kch1 (a Kamchatka isolate) and Aliivibrio salmonicida
    Manukhov, I.V.; Khrul’mova, S.A.; Baranova, A.; Zavilgelsky, G.B.
  • Role of P. aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes
    Pearson, J.P.; Pesei, E.C.; Iglewski, B.H.
  • P. aeruginosa quorum- sensing systems and virulence
    Smith, R.S.; Iglewski, B.H.P.
  • Regulation of microcin C51 operon expression: the role of global regulators of transcription
    Fomenko, D.; Veselowskii, A.; Khmel, I.A.
  • Photorhabdus luminescens luxCDABE promoter probe vectors
    Dyk, T.K.; Rosson, R.A.
  • Trigger factor from psychrophilic bacterium Psychrobacter frigidicola is a monomeric chaperone
    Robin, S.; Togashi, D.; Ryder, A.G.; Wall, J.G.
  • The C-terminal domain of the Vibrio fischeri transcription activator LuxR is not essential for degradation by Lon protease
    Mel’kina, O.E.; Manukhov, I.V.; Zavilgelsky, G.B.
  • Involvement of host factors in the regulation of the Vibrio fischeri lux operon in Escherichia coli cells
    Manukhov, I.V.; Kotova, V.Yu.; Zavilgelsky, G.B.
  • Lux-operon of the marine psychrophilic bacteria Aliivibrio logei: a comparative analysis of the LuxR1/LuxR2 regulatory activity in Escherichia coli cells
    Khrulnova, S.A.; Baranova, A.; Bazhenov, S.V.
  • Effect of sdiA on biosensors of N-acylhomoserine-lactones
    Lindsay, A.; Ahmer, B.M.M.
  • Antimodification activity of the ArdA and Ocr proteins
    Zavilgelskii, G.B.; Kotova, V.Yu.; Rastorguev, S.M.
  • The novel gene(s) ARD of plasmid pKM101: alleviation of EcoK restriction
    Belogurov, A.A.; Yusiffov, T.N.; Kotova, V.Yu.; Zavilgelsky, G.B.
  • Nucleotide sequence of the gene (ard) encoding the antirestriction protein of plasmid ColIb-P9
    Delver, E.P.; Kotova, V.Yu.; Zavilgelsky, G.B.; Belogurov, A.A.
  • Extensive DNA mimicry by the ArdA anti-restriction protein and its role in the spread of antibiotic resistance
    McMahon, S.A.; Roberts, G.A.; Johnson, K.A.
  • Selective silencing of foreign DNA with low GC content by the H-NS protein in Salmonella
    Navarre, W.W.; Porwollik, S.; Wang, Y.
  • Structural basis for recognition of AT-rich DNA by unrelated xenogenic silencing proteins
    Gordon, B.R.; Li, Y.; Cote, A.

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial