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221 62 62 3 3 G. Wolf G. Schünzel F. Rothe Institute of Biology Medical Academy Magdeburg Erich-Weinert-Str.3 DDR-3014 Magdeburg German Democratic Republic Summary Using a special tetrazolium salt technique, a striking correlation was observed between Na + concentration of the incubation medium and the formation of formazan catalyzed by glutamate dehydrogenase (GDH) in glutaniatergic neuropil areas of the hippocampal formation, cerebellum, and other brain regions. Na + concentrations of 130–150 mmol/l caused maximal formazan production. The GDH catalyzed sodium-dependent increase in formazan production is suggested to be a consequence of the sodium dependence of glutamate uptake in glutaniatergic brain structures supplying the enzyme with substrate.
Experimental Brain Research – Springer Journals
Published: May 1, 1986
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