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- Publisher
- Springer Journals
- Copyright
- Copyright © 2010 by Pleiades Publishing, Ltd.
- Subject
- Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
- ISSN
- 1022-7954
- eISSN
- 1608-3369
- DOI
- 10.1134/S1022795410120070
- Publisher site
- See Article on Publisher Site
Abstract
A novel antifungal peptide, LAMP-1a, was isolated from sand-elymus (Leymus arenarius) seeds. Expression of a synthetic gene encoding this peptide in Escherichia coli cells was obtained. The target peptide was expressed as a fusion with thioredoxin. Identity of the recombinant peptide to native LAMP-1a was confirmed by chromatography, mass spectrometry, and amino acid sequencing. LAMP-1a displayed a high inhibitory activity in respect of a number of phytopathogenic fungi in in vitro assays, which opens up possibilities for the gene encoding it to be used for genetic transformation of plants and for engineering pathogen-resistant crops
Journal
Russian Journal of Genetics – Springer Journals
Published: Dec 16, 2010