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- Publisher
- Springer Journals
- Copyright
- Copyright © 2011 by Pleiades Publishing, Ltd.
- Subject
- Life Sciences; Biochemistry, general; Medical Microbiology ; Microbiology
- ISSN
- 0003-6838
- eISSN
- 1608-3024
- DOI
- 10.1134/S0003683811040193
- Publisher site
- See Article on Publisher Site
Abstract
Bacillus licheniformis WBL-3, one of poly-γ-glutamic acid (γ-PGA) producers, depends on the existence of glutamate in the medium. In this paper, γ-PGA synthetase complex gene (pgsBCA) was cloned from Bacillus licheniformis WBL-3. pgsBCA gene of B. licheniformis WBL-3 was highly homologous with pgs-BCA gene of B. licheniformis 14580. The similarity was 97%, but the similarity of pgsBCA gene between B. licheniformis WBL-3 and Bacillus subtilis IFO3336 was only 74%. However, when pgsBCA was expressed in Escherichia coli, the E. coli clone produced γ-PGA extracellularly. The yield of γ-PGA was 8.624 g/l. This result infers that B. licheniformis and B. subtilis has the similar γ-PGA biosynthesis mechanism, namely, glutamic acid is catalyzed by an ATP-dependent amide ligase to synthesize γ-PGA.
Journal
Applied Biochemistry and Microbiology – Springer Journals
Published: Jul 5, 2011