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P. Charest, L. Holbrook, J. Gabard, V. Iyer, B. Miki (1988)
Agrobacterium-mediated transformation of thin cell layer explants from Brassica napus L.Theoretical and Applied Genetics, 75
J. Fry, A. Barnason, R. Horsch (1987)
Transformation of Brassica napus with Agrobacterium tumefaciens based vectorsPlant Cell Reports, 6
L. Holbrook, B. Miki (1985)
Brassica grown gall tumourigenesis and in vitro of transformed tissuePlant Cell Reports, 4
A. Hoekema, P. Hirsch, P. Hooykaas, R. Schilperoort (1983)
A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 303
G. An, B. Watson, C. Chiang (1986)
Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System.Plant physiology, 81 1
F. Hoffmann, R. Sibley, S. Tsay (1988)
Transgenic antibiotic resistance may be differentially silenced in germinating pollen grainsPlant Cell Reports, 7
E. Swanson, M. Coumans, Sau‐Ching Wu, T. Barsby, W. Beversdorf (1987)
Efficient isolation of microspores and the production of microspore-derived embryos from Brassica napusPlant Cell Reports, 6
E. Pua, A. Mehra-Palta, F. Nagy, N. Chua (1987)
Transgenic Plants of Brassica napus L.Bio/Technology, 5
E. Swanson, M. Coumans, Gerry Brown, J. Patel, W. Beversdorf (1988)
The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplastsPlant Cell Reports, 7
S. Radke, B. Andrews, M. Moloney, M. Crouch, J. Kridl, V. Knauf (1988)
Transformation of Brassica napus L. using Agrobacterium tumefaciens: developmentally regulated expression of a reintroduced napin geneTheoretical and Applied Genetics, 75
R. Slater (2014)
Experiments in Molecular Biology
M. Bevan (1984)
Binary Agrobacterium vectors for plant transformation.Nucleic acids research, 12 22
S. Stachel, E. Messens, M. Montagu, P. Zambryski (1985)
Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciensNature, 318
E. Swanson (1990)
Microspore culture in brassica.Methods in molecular biology, 6
R. Bino, J. Hille, J. Franken (1987)
Kanamycin resistance during in vitro development of pollen from transgenic tomato plantsPlant Cell Reports, 6
(1987)
A rapid whole bud maceration technique for the efficient isolation of microspore-derived embryos in Brass# ca
G. Neuhaus, G. Spangenberg, O. Scheid, H. Schweiger (1987)
Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoidsTheoretical and Applied Genetics, 75
122 78 78 6 6 E. B. Swanson L. R. Erickson Allelix Crop Technologies 6850 Goreway Drive L4V1P1 Mississauga Ontario Canada Summary Microspore-derived embryos of Brassica napus were transformed using the disarmed octopine-producing LBA4404 strain of Agrobacterium tumefaciens containing the binary vector pBin19. Octopine-producing strains have previously been reported to be ineffective in transforming Brassica . Four actively growing yellow/ green sectors were selected from the embryos on 50 mg/l kanamycin and plants regenerated. Analysis for NPT-II activity in these young plants initially indicated no expression of the bacterial NPT-II gene. The plants were nevertheless grown to maturity, selfed and S 1 seed was collected. Three of the S 1 plants produced microspores which were from 4 to 20 times more tolerant to kanamycin than the original parent. Southern analysis revealed that one plant (EC-1) had a single site of insertion and the other two plants (EC-2 and EC-6) had two sites of insertion with sequence homology to the bacterial NPT-II gene. Microspores from the EC-2 and EC-6 transgenics produced embryos on approximately five times the level of kanamycin tolerated by microspores from untransformed plants, while the EC-1 transgenic produced microspores with more than 20 times the tolerance to kanamycin. Analysis of S 1 progeny of the EC-1 transgenic indicated that 100% of the progeny exhibited the trait through both Southern analysis and by expressing tolerance to kanamycin in microspore-derived embryos.
TAG Theoretical and Applied Genetics – Springer Journals
Published: Dec 1, 1989
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