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Gluconobacter oxydans NAD-dependent, D -fructose reducing, polyol dehydrogenases activity: screening, medium optimisation and application for enzymatic polyol production



Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l −1 , 7.2 g peptone l−1 and 1.8 g yeast extract l −1 . Two D -fructose reducing, NAD-dependent intracellular enzymes were present in the G. oxydans cell-free extract: sorbitol dehydrogenase, and mannitol dehydrogenase. Substrate reduction occurred optimally at a low pH (pH 6), while the optimum for substrate oxidation was situated at alkaline pHs (pH 9.5–10.5). The mannitol dehydrogenase was more thermostable than the sorbitol dehydrogenase. The cell-free extract could be used to produce D -mannitol and D -sorbitol enzymatically from D -fructose. Efficient coenzyme regeneration was accomplished by formate dehydrogenase-mediated oxidation of formate into CO 2 .



Biotechnology LettersSpringer Journals

Published: Mar 1, 2005

DOI: 10.1007/s10529-005-0684-6

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