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Full-Length Normalization Subtractive Hybridization: A Novel Method for Generating Differentially Expressed cDNAs

Full-Length Normalization Subtractive Hybridization: A Novel Method for Generating Differentially... Here we present a novel method termed full-length normalization subtractive hybridization (FNSH) for efficiently generating subtracted cDNA libraries with a high degree of productivity. This method has the ability to isolate full-length differentially expressed genes from target samples. Normalization and subtraction of FNSH are performed simultaneously with efficiency equal to or even higher than that of suppression subtractive hybridization. Using FNSH, we have isolated at least 40 unique cDNAs that are expressed in terminal ampullae but not in the ovaries of the prawn Macrobrachium rosenbergii from 120 randomly picked subtracted clones. Sequence analysis shows that 37 of the 40 cDNAs are full length. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Biotechnology Springer Journals

Full-Length Normalization Subtractive Hybridization: A Novel Method for Generating Differentially Expressed cDNAs

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References (10)

Publisher
Springer Journals
Copyright
Copyright © 2009 by Humana Press
Subject
Chemistry; Human Genetics ; Biological Techniques; Protein Science; Cell Biology; Biochemistry, general; Biotechnology
ISSN
1073-6085
eISSN
1559-0305
DOI
10.1007/s12033-009-9198-0
pmid
19669953
Publisher site
See Article on Publisher Site

Abstract

Here we present a novel method termed full-length normalization subtractive hybridization (FNSH) for efficiently generating subtracted cDNA libraries with a high degree of productivity. This method has the ability to isolate full-length differentially expressed genes from target samples. Normalization and subtraction of FNSH are performed simultaneously with efficiency equal to or even higher than that of suppression subtractive hybridization. Using FNSH, we have isolated at least 40 unique cDNAs that are expressed in terminal ampullae but not in the ovaries of the prawn Macrobrachium rosenbergii from 120 randomly picked subtracted clones. Sequence analysis shows that 37 of the 40 cDNAs are full length.

Journal

Molecular BiotechnologySpringer Journals

Published: Aug 11, 2009

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