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Fluorimetric reaction detectors for high-performance liquid chromatography

Fluorimetric reaction detectors for high-performance liquid chromatography Using selected steroids proposals are investigated for coupling liquid chromatographic separations with derivatization after separation for fluorimetric detection. Reverse-phase-, adsorption chromatography, systems with chemically bonded phases as well as one-step reaction with isonicotinoylhydrazide in organic solvents and a multistep reaction of ketosteroids to form dihydrolutidine derivatives in aqueous solution were combined. The effects of the optimized reaction conditions in an air-segmented mixing coil reactor and the mobile phase on fluorescence yield and detection limit are exemplified and discussed. The detection limits for corticosterone are 7 resp. 100 ng in the reactor, when connected to a HPLC-system 100 resp. 500 ng (one-step resp. multi-step reaction). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

Fluorimetric reaction detectors for high-performance liquid chromatography

Schwedt, G.; Reh, E.
Chromatographia , Volume 14 (4) – Apr 1, 1981
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References (11)

Publisher
Springer Journals
Copyright
Copyright © 1981 by Friedr. Vieweg & Sohn Verlagsgesellschaft mbH
Subject
Chemistry; Analytical Chemistry; Organic Chemistry; Biochemistry, general; Plant Sciences; Pharmacy; Measurement Science, Instrumentation
ISSN
0009-5893
eISSN
1612-1112
DOI
10.1007/BF02260160
Publisher site
See Article on Publisher Site

Abstract

Using selected steroids proposals are investigated for coupling liquid chromatographic separations with derivatization after separation for fluorimetric detection. Reverse-phase-, adsorption chromatography, systems with chemically bonded phases as well as one-step reaction with isonicotinoylhydrazide in organic solvents and a multistep reaction of ketosteroids to form dihydrolutidine derivatives in aqueous solution were combined. The effects of the optimized reaction conditions in an air-segmented mixing coil reactor and the mobile phase on fluorescence yield and detection limit are exemplified and discussed. The detection limits for corticosterone are 7 resp. 100 ng in the reactor, when connected to a HPLC-system 100 resp. 500 ng (one-step resp. multi-step reaction).

Journal

Chromatographia – Springer Journals

Published: Apr 1, 1981

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