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First infectious clone of the propagatively transmitted Oat blue dwarf virus

First infectious clone of the propagatively transmitted Oat blue dwarf virus Oat blue dwarf virus (OBDV) is a small, phloem-limited marafivirus that replicates in its leafhopper vector. We have developed complete cDNA clones of OBDV from which infectious transcripts may be derived––the first such clones for any propagatively transmitted plant virus. Prior to clone construction, the reported sequences of the 5′ and 3′ ends were confirmed using 5′ RACE, primer extension, and ligation-anchored PCR. Using vascular puncture of maize seeds with capped transcripts, multiple clones were shown to be infectious at an average rate of 24.3% (range 14–36%). Aster leafhoppers successfully transmitted OBDV to oats and barley after feeding on detached, infected maize leaves. Proteins and RNAs consistent in size with those expected in OBDV infection were detected in young leaves via western and northern blotting, respectively. One construct, pOBDV-2r, was designated as the reference clone. An infectious clone of OBDV will be valuable in examining the interaction of this virus with both its insect and plant hosts. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

First infectious clone of the propagatively transmitted Oat blue dwarf virus

Edwards, Michael; Weiland, John
Archives of Virology , Volume 155 (4) – Apr 1, 2010
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References (30)

Publisher
Springer Journals
Copyright
Copyright © 2010 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
DOI
10.1007/s00705-010-0603-6
pmid
20224895
Publisher site
See Article on Publisher Site

Abstract

Oat blue dwarf virus (OBDV) is a small, phloem-limited marafivirus that replicates in its leafhopper vector. We have developed complete cDNA clones of OBDV from which infectious transcripts may be derived––the first such clones for any propagatively transmitted plant virus. Prior to clone construction, the reported sequences of the 5′ and 3′ ends were confirmed using 5′ RACE, primer extension, and ligation-anchored PCR. Using vascular puncture of maize seeds with capped transcripts, multiple clones were shown to be infectious at an average rate of 24.3% (range 14–36%). Aster leafhoppers successfully transmitted OBDV to oats and barley after feeding on detached, infected maize leaves. Proteins and RNAs consistent in size with those expected in OBDV infection were detected in young leaves via western and northern blotting, respectively. One construct, pOBDV-2r, was designated as the reference clone. An infectious clone of OBDV will be valuable in examining the interaction of this virus with both its insect and plant hosts.

Journal

Archives of Virology – Springer Journals

Published: Apr 1, 2010

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