Keywords Antioxidant activity Butterﬂy pea Clitoria ternatea Extractive effect of solvent Flavonoid HPLC analysis Introduction Methods for extraction of phenolic compounds from plant sources vary widely due to the complex nature of the sample matrix and because different plant materials contain different forms of ﬂavonoid compounds . A natural source with high phenolic content and high biological activity is a prerequisite for industrial-scale production. Phytochemical yield and antioxidant capacity are signiﬁcantly inﬂu- enced by the solvents used for extraction, due to their varying dielectric properties and extraction efﬁciency, as well as the structural variability of the compounds targeted in the process [2, 3]. Traditional heating reﬂux is widely used for extraction of phenolics from plant sources because of its easy operation. However, proper selection of solvents, optimum solid-to-liquid ratio, and duration and temperature of extraction is imperative to achieve high efﬁciency. Hence, such procedures should be standardized for maximum extraction to provide the real composition and optimal level of ﬂavonoids from plants [1, 4]. Flavonoids are the most widely distributed, ubiquitous plant secondary metabolites, mainly existing in the form of glycoside conjugates. Their chromatographic analysis generates a large number of peaks, making individual ﬂavonoid
Research on Chemical Intermediates – Springer Journals
Published: Jul 18, 2016
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