Access the full text.
Sign up today, get DeepDyve free for 14 days.
Y. Zhang (2014)
10.1007/s00253-013-4881-2Appl. Microbiol. Biotechnol., 98
A. Cuesta (2011)
10.1016/j.molimm.2011.03.022Mol. Immunol., 48
T. Ganz (2003)
10.1038/nri1180Nat. Rev. Immunol., 3
T. Liang (2013)
10.1016/j.cbpb.2013.07.006Comp. Biochem. Physiol. B., 166
M. Guo (2012)
10.1016/j.fsi.2012.02.005Fish Shellfish Immunol., 32
Z. Peng (2014)
10.1007/s00253-014-5560-7Appl. Microbiol. Biotechnol., 98
X.T. Cao (2015)
10.1007/s00253-014-6077-9Appl. Microbiol. Biotechnol., 99
G. Diamond (1991)
10.1073/pnas.88.9.3952Proc. Natl. Acad. Sci., 88
Z. Chen (2011)
10.1007/s00253-010-2864-0Appl. Microbiol. Biotechnol., 89
M.E. Selsted (1993)
10.1016/S0021-9258(18)53298-1J. Biol. Chem., 268
J. Zou (2007)
10.1016/j.molimm.2006.01.012Mol. Immunol., 44
H. Schägger (1987)
10.1016/0003-2697(87)90587-2Anal. Biochem., 166
T. Ganz (1985)
10.1172/JCI112120J. Clin. Invest., 76
P. Kant (2009)
10.1016/j.peptides.2009.05.024Peptides, 30
B.E. Beck–Broichsitter (2015)
10.1111/jop.12202J. Oral. Pathol. Med., 44
G. Wang (2012)
10.1016/j.fsi.2012.06.003Fish Shellfish Immunol., 33
J. Harder (2001)
10.1074/jbc.M008557200J. Biol. Chem., 276
K.H. Hsu (2009)
10.2323/jgam.55.395J. Gen. Appl. Microbiol., 55
M.E. Selsted (1985)
10.1172/JCI112121J. Clin. Invest., 76
A. Wang (2009)
10.1007/s00253-009-2020-xAppl. Microbiol. Biotechnol., 84
Y. Yamaguchi (2002)
10.4049/jimmunol.169.5.2516J. Immunol., 169
Defensins are abundant in cells and tissues that are involved in host defense against microbial infections. zfDB3 (zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish (Danio rerio). Here we focus on mzfDB3, which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized mzfDB3 gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into Pichia pastoris X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide (rmzfDB3) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product (rmzfDB3, 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed antibacterial activity against Grampositive (i.e., Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus) and Gram-negative (i.e., Escherchia coli BL21, Vibrio parahaemolyticus, Salmonella lignieres, and Pseudomonas aeruginosa) bacteria.
Applied Biochemistry and Microbiology – Springer Journals
Published: Nov 23, 2017
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.