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Expression of the Xylanase Gene from Paenibacillus brasilensis X1 in Pichia pastoris and Characteristics of the Recombinant Enzyme

Expression of the Xylanase Gene from Paenibacillus brasilensis X1 in Pichia pastoris and... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 8, pp. 797–804. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2018, published in Biotekhnologiya, 2018, Vol. 34, No. 6, pp. 22–32. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Expression of the Xylanase Gene from Paenibacillus brasilensis X1 in Pichia pastoris and Characteristics of the Recombinant Enzyme a, a a A. N. Kalinina *, T. L. Gordeeva , and S. P. Sineoky State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GOSNIIGENETIKA, Kurchatov Institute NRC), Moscow, 117545 Russia *e-mail: anna.kalininaa@yandex.ru Received October 12, 2018; revised November 15, 2018; accepted December 15, 2018 Abstract—The heterologous expression, isolation, and characterization of a novel xylanase from Paenibacillus brasilensis are described. The xyl1 gene from the Paenibacillus brasilensis strain X1 VKPM B-13092, which consists of 639 nucleotides, encodes a secreted endo-1,4-β-xylanase (EC 3.2.1.8) containing 184 amino acids and 28 residues of the putative signal peptide in the N-terminal region. The nucleotide sequence of the xyl1 gene and the amino acid sequence of the mature Xyll protein have the greatest homology with the Bacillus subtilis endo-1,4-β-xylanase sequences (78 and 83%, respectively). A gene fragment encoding the mature http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Expression of the Xylanase Gene from Paenibacillus brasilensis X1 in Pichia pastoris and Characteristics of the Recombinant Enzyme

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References (49)

Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819080064
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 8, pp. 797–804. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2018, published in Biotekhnologiya, 2018, Vol. 34, No. 6, pp. 22–32. PRODUCERS, BIOLOGY, SELECTION, AND GENE ENGINEERING Expression of the Xylanase Gene from Paenibacillus brasilensis X1 in Pichia pastoris and Characteristics of the Recombinant Enzyme a, a a A. N. Kalinina *, T. L. Gordeeva , and S. P. Sineoky State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (GOSNIIGENETIKA, Kurchatov Institute NRC), Moscow, 117545 Russia *e-mail: anna.kalininaa@yandex.ru Received October 12, 2018; revised November 15, 2018; accepted December 15, 2018 Abstract—The heterologous expression, isolation, and characterization of a novel xylanase from Paenibacillus brasilensis are described. The xyl1 gene from the Paenibacillus brasilensis strain X1 VKPM B-13092, which consists of 639 nucleotides, encodes a secreted endo-1,4-β-xylanase (EC 3.2.1.8) containing 184 amino acids and 28 residues of the putative signal peptide in the N-terminal region. The nucleotide sequence of the xyl1 gene and the amino acid sequence of the mature Xyll protein have the greatest homology with the Bacillus subtilis endo-1,4-β-xylanase sequences (78 and 83%, respectively). A gene fragment encoding the mature

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Dec 5, 2019

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