Access the full text.
Sign up today, get DeepDyve free for 14 days.
CR Denz, C Zhang, P Jia, J Du, X Huang, S Dube, A Thomas, BJ Poiesz, DK Dube (2011)
Absence of mutation at the 5′-upstream promoter region of the TPM4 gene from cardiac mutant axolotl (Ambystoma mexicanum)Cardiovascular Toxicology, 11
S Hardy, N Theze, D Lepetit, MR Allo, P Thiebaud (1995)
The Xenopus laevis TM-4 gene encodes non-muscle and cardiac tropomyosin isoforms through alternative splicingGene, 156
J Du, C Nan, JJ Huang, C Zhang, J Liu, P Jia, M Abers, XP Huang (2008)
Functional characterization of mouse fetal TnI gene promoters in myocardial cellsJournal of Biomedical Science, 15
CY Chen, RJ Schwartz (1996)
Recruitment of the tinman homolog Nkx-2.5 by serum response factor activates cardiac alpha-actin gene transcriptionMolecular and Cellular Biology, 16
S Pinnamaneni, S Dube, C Welch, R Shrestha, PM Benz, L Abbott, BJ Poiesz, DK Dube (2013)
Effect of Shz-1, a cardiogenic small molecule, on expression of tropomyosin in axolotl heartAmerican Based Research Journal, 2
G Schevzov, S Whittaker, T Fath, JJ-C Lin, PW Gunning (2011)
Tropomyosin isoforms and reagentsBioArchitecture, 1
MW Pffaffl (2001)
A new mathematical model for relative quantification in real time RT-PCRNucleic Acids Research, 29
K Piples, DF Wieczorek (2000)
Tropomyosin 3 increases striated muscle isoform diversityBiochemistry, 39
C Zhang, P Jia, X Huang, GF Sferrazza, G Athauda, AP Achary, J Wang, SL Lemanski, DK Dube, LF Lemanski (2009)
Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl heartsJournal of Biomedical Sciences, 16
A Thomas, S Rajan, HL Thurston, SN Masineni, P Dube, A Bose, V Muthu, S Dube, DF Wieczorek, BJ Poiesz, DK Dube (2010)
Expression of a novel tropomyosin isoform in axolotl heart and skeletal muscleJournal of Cellular Biochemistry, 110
H Sadek, B Hannack, E Choe, J Wang, S Latif, MG Garry, G Mary, DJ Garry, J Longgood, DE Frantz, EN Olson, J Hsieh, JW Schneider (2008)
Cardiogenic small molecules that enhance myocardial repair by stem cellsProceedings of the National Academy of Sciences USA, 105
MF Pittenger, JA Kazzaz, DM Helfman (1994)
Functional properties of nonmuscle tropomyosin isoformsCurrent Opinion in Cell Biology, 6
P Gunning, G O’Neill, E Hardmen (2008)
Tropomyosin-based regulation of actin cytoskeleton in time and spacePhysiology Review, 88
BJ Spinner, RW Zajdel, MD McLean, CR Denz, S Dube, S Mehta, A Choudhury, M Nakatsugawa, N Dobbins, LF Lemanski, DK Dube (2002)
Characterization of a TM-4 type tropomyosin that is essential for myofibrillogenesis and contractile activity in embryonic hearts of the Mexican axolotlJournal of Cellular Biochemistry, 85
LF Lemanski (1979)
Role of tropomyosin in actin filament formation in embryonic salamander heart cellsJournal of Cell Biology, 82
D Yaffe, O Saxel (1977)
Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscleNature, 270
RW Zajdel, MD McLean, SL Lemanski, M Muthuchamy, DF Wieczorek, LF Lemanski, DK Dube (1998)
Ectopic expression of tropomyosin promotes myofibrillogenesis in mutant axolotl heartsDevelopmental Dynamics, 213
KJ Livak, TD Schmittgen (2001)
Analysis of relative gene expression data using real-time quantitative PCR and 2-DDCT methodMethods, 25
M Tanaka, H Kasahara, S Bartunkova, M Schinke, I Komuro, H Inagaki, Y Lee, G Lyons, S Izumo (1998)
Vertebrate homologs of tinman and bagpipe: Roles of the homeobox genes in cardiovascular developmentDevelopmental Genetics, 22
HL Thurston, S Prayaga, A Thomas, V Guharoy, S Dube, BJ Poiesz, DK Dube (2009)
Expression of Nkx2.5 in wild type, cardiac mutant, and thyroxine-induced metamorphosed hearts of the Mexican axolotlCardiovascular Toxicology, 9
DE Fleenor, KH Hickman, GJ Lindquester, RB Devlin (1992)
Avian cardiac tropomyosin gene produces tissue-specific isoforms through alternative RNA splicingJournal of Muscle Research & Cell Motility, 13
EA Luque, LF Lemanski, DK Dube (1994)
Molecular cloning, sequencing and expression of a tropomyosin form cardiac muscle of the Mexican axolotl, Ambystoma mexicanumBiochemical and Biophysical Research Communications, 203
RW Zajdel, MD McLean, LF Lemanski, DK Dube (2000)
Alteration of cardiac myofibrillogenesis by lipofectin-mediated delivery of exogenous proteins and nucleic acids into whole embryonic heartsAnatomy and Embryology, 210
AM Riazi, H Lee, C Hsu, GV Arsdell (2005)
CSX/Nkx2.5 modulates differentiation of skeletal myoblasts and promotes differentiation into neuronal cells in vitroJournal of Biological Chemistry, 280
RW Zajdel, DK Dube, LF Lemanski (1999)
The cardiac mutant Mexican axolotl is a unique animal model for evaluation of cardiac myofibrillogenesisExperimental Cell Research, 248
EA Luque, BJ Spinner, S Dube, DK Dube, LF Lemanski (1997)
Differential expression of a novel isoform of alpha-tropomyosin in cardiac and skeletal muscle of the Mexican axolotl (Ambystoma mexicanum)Gene, 185
RJ Schwartz, J Sepulveda, SN Belaguli (2002)
Myofibrillogenesis
CF Clemente, MA Corat, ST Saad, KG Franchini (2005)
Differentiation of C2C12 myoblasts is critically regulated by FAK signalingAmerican Journal of Physiology: Regulatory, Integrative and Comparative Physiology, 289
SV Perry (2001)
Vertebrate tropomyosin, properties and functionJournal of Muscle Research and Cell Motility, 22
C Nan, X Huang (2009)
Transcription factor Yin Yang 1 represses fetal troponin I gene expression in neonatal myocardial cellsBiochemical and Biophysical Research Communications, 378
DF Wieczorek (1988)
Regulation of alternatively spliced alpha-tropomyosin gene expression by nerve extractJournal of Biological Chemistry, 263
J Lees-Miller, D Helfman (1991)
The molecular basis for tropomyosin isoform diversityBioEssays, 13
C Biben, RP Harvey (1997)
Homeodomain factor Nk2–5 controls left/right asymmetric expression of bHLH gene eHand during murine heart developmentGenes & Development, 11
JS Yuan, A Reed, F Chen, CN Stewart (2006)
Statistical analysis of real-time PCR dataBMC Bioinformatics, 7
We evaluated the effect of shz-1, a cardiogenic molecule, on the expression of various tropomyosin (TM) isoforms in the Mexican axolotl (Ambystoma mexicanum) hearts. qRT-PCR data show a ~1.5-fold increase in cardiac transcripts of the Nkx2.5 gene, which plays a crucial role in cardiogenesis in vertebrates. Shz-1 augments the expression of transcripts of the total sarcomeric TPM1 (both TPM1α & TPM1κ) and sarcomeric TPM4α. In order to understand the mechanism by which shz-1 augments the expression of sarcomeric TPM transcription in axolotl hearts, we transfected C2C12 cells with pGL3.axolotl. We transfected C2C12 cells with pGL3-axolotl TPM4 promoter constructs containing the firefly luciferase reporter gene. The transfected C2C12 cells were grown in the absence or presence of shz-1 (5 μM). Subsequently, we determined the firefly luciferase activity in the extracts of transfected cells. The results suggest that shz-1 activates the axolotl TPM4 promoter-driven ectopic expression in C2C12 cells. Also, we transfected C2C12 cells with a pGL3.1 vector containing the promoter of the mouse skeletal muscle troponin-I and observed a similar increase in the luciferase activity in shz-1-treated cells. We conclude that shz-1 activates the promoters of a variety of genes including axolotl TPM4. We have quantified the expression of the total sarcomeric TPM1 and observed a 1.5-fold increase in treated cells. Western blot analyses with CH1 monoclonal antibody specific for sarcomeric isoforms show that shz-1 does not increase the expression of TM protein in axolotl hearts, whereas it does in C2C12 cells. These findings support our hypothesis that cardiac TM expression in axolotl undergoes translational control.
Cardiovascular Toxicology – Springer Journals
Published: Jun 24, 2014
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.