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Expression of pisatin demethylating ability in Nectria haematococca

Expression of pisatin demethylating ability in Nectria haematococca 203 129 129 1 1 Hans D. Van Etten Wolfgang Barz Department of Plant Pathology Cornell University 14853 Ithaca New York USA Lehrstuhl für Biochemie der Pflanzen Westfälische Wilhelms-Universität D-4400 Münster Germany Abstract A mycelial suspension of Nectria haematococca completely demethylated 0.1 mM pisatin in 2 h in a medium free of other carbon sources while no demethylation of pisatin by the fungus occurred in 6 h when 2% glucose was in the medium. Prior exposure of the fungal cells to pisatin in glucosefree medium markedly enhanced the rate of pisatin demethylation, with maximum stimulation occurring 5–9h after the initial exposure. The stimulation of pisatin demethylating ability was relatively specific for pisatin as the inducer. Out of a large variety of isoflavonoids tested the only compounds other than pisatin that stimulated the activity significantly were pterocarpan or isoflavan derivatives. Protoplasts with pisatin demethylating ability were isolated from pisatin-treated mycelium. Attempts to obtain a cell-free system with pisatin demethylating ability from these protoplasts were unsuccessful. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Expression of pisatin demethylating ability in Nectria haematococca

Etten, Hans; Barz, Wolfgang
Archives of Microbiology , Volume 129 (1) – Mar 1, 1981
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References (24)

Publisher
Springer Journals
Copyright
Copyright © 1981 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00417181
Publisher site
See Article on Publisher Site

Abstract

203 129 129 1 1 Hans D. Van Etten Wolfgang Barz Department of Plant Pathology Cornell University 14853 Ithaca New York USA Lehrstuhl für Biochemie der Pflanzen Westfälische Wilhelms-Universität D-4400 Münster Germany Abstract A mycelial suspension of Nectria haematococca completely demethylated 0.1 mM pisatin in 2 h in a medium free of other carbon sources while no demethylation of pisatin by the fungus occurred in 6 h when 2% glucose was in the medium. Prior exposure of the fungal cells to pisatin in glucosefree medium markedly enhanced the rate of pisatin demethylation, with maximum stimulation occurring 5–9h after the initial exposure. The stimulation of pisatin demethylating ability was relatively specific for pisatin as the inducer. Out of a large variety of isoflavonoids tested the only compounds other than pisatin that stimulated the activity significantly were pterocarpan or isoflavan derivatives. Protoplasts with pisatin demethylating ability were isolated from pisatin-treated mycelium. Attempts to obtain a cell-free system with pisatin demethylating ability from these protoplasts were unsuccessful.

Journal

Archives of Microbiology – Springer Journals

Published: Mar 1, 1981

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