Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Expression and localization of bacterial luciferase determined by immunogold labeling

Expression and localization of bacterial luciferase determined by immunogold labeling 203 152 152 1 1 Pio Colepicolo Marie-Thérèse Nicolas Jean-Marie Bassot J. Woodland Hastings Department of Cellular and Developmental Biology Harvard University 02138 Cambridge MA USA Laboratoire de Bioluminescence CNRS 105 Blvd Raspail 75006 Paris France Abstract Using a polyclonal antibody raised against purified luciferase of Vibrio harveyi and immunogold labeling on thin sections, the amounts and cellular localization of luciferase were examined during the growth of the bacteria. Cells harvested at different times during cultivation in liquid medium at 22°C were fixed, either chemically or by fast freeze fixation followed by freeze substitution, and embedded in LR White. Concomitant measures of bioluminescence, both in vitro and in vivo,showed the classical curve of autoinduction. The number of gold particles per cell area showed a similar pattern. Their localization was always cytoplasmic, with no indication of special periplasmic or membrane associations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Expression and localization of bacterial luciferase determined by immunogold labeling

Loading next page...
 
/lp/springer-journals/expression-and-localization-of-bacterial-luciferase-determined-by-07jkSKfRxU

References (26)

Publisher
Springer Journals
Copyright
Copyright © 1989 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00447014
Publisher site
See Article on Publisher Site

Abstract

203 152 152 1 1 Pio Colepicolo Marie-Thérèse Nicolas Jean-Marie Bassot J. Woodland Hastings Department of Cellular and Developmental Biology Harvard University 02138 Cambridge MA USA Laboratoire de Bioluminescence CNRS 105 Blvd Raspail 75006 Paris France Abstract Using a polyclonal antibody raised against purified luciferase of Vibrio harveyi and immunogold labeling on thin sections, the amounts and cellular localization of luciferase were examined during the growth of the bacteria. Cells harvested at different times during cultivation in liquid medium at 22°C were fixed, either chemically or by fast freeze fixation followed by freeze substitution, and embedded in LR White. Concomitant measures of bioluminescence, both in vitro and in vivo,showed the classical curve of autoinduction. The number of gold particles per cell area showed a similar pattern. Their localization was always cytoplasmic, with no indication of special periplasmic or membrane associations.

Journal

Archives of MicrobiologySpringer Journals

Published: Jun 1, 1989

There are no references for this article.