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J. Escaig (1982)
New instruments which facilitate rapid freezing at 83 K and 6 KJournal of Microscopy, 126
J. Hastings, T. Baldwin, Nicoli Mz (1978)
[14] Bacterial luciferase: Assay, purification, and propertiesMethods in Enzymology, 57
J. Mey (1983)
6 – Colloidal Gold Probes in Immunocytochemistry
J. Hastings, Catherine Potrikusv, Subhash Gupta, M. Kurfürst, J. Makemson (1985)
Biochemistry and physiology of bioluminescent bacteria.Advances in microbial physiology, 26
J. Hastings, G. Weber (1963)
Total Quantum Flux of Isotropic SourcesJournal of the Optical Society of America, 53
(1971)
A stable, inexpensive, solid-state
T. Kehle, V. Herzog (1987)
Interactions between protein-gold complexes and cell surfaces: a method for precise quantitation.European journal of cell biology, 45 1
J. Hastings (1986)
Bioluminescence in Bacteria and Dinoflagellates
S. Shall (1987)
Cellular controlNature, 330
(1987)
Interactions between protein-gold
G. Newman, B. Jasani, E. Williams (1982)
THE PRESERVATION OF ULTRASTRUCTURE AND ANTIGENICITYJournal of Microscopy, 127
M. Bendayan, A. Nanci, F. Kan (1987)
Effect of tissue processing on colloidal gold cytochemistry.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35
R. Rosson, K. Nealson (1981)
Autoinduction of bacterial bioluminescence in a carbon limited chemostatArchives of Microbiology, 129
C. Waters, J. Hastings (1977)
Mutants of luminous bacteria with an altered control of luciferase synthesisJournal of Bacteriology, 131
C. Reeve, T. Baldwin (1982)
Synthesis and inactivation of bacterial luciferase determined by immunochemical techniques. Comparison with total protein synthesis and turnover.The Journal of biological chemistry, 257 2
(1977)
A glycoprotein with luciferase activity isolated from Photobacterium leiognathi
K. Nealson, T. Platt, J. Hastings (1970)
Cellular Control of the Synthesis and Activity of the Bacterial Luminescent SystemJournal of Bacteriology, 104
(1978)
Isolation , identification and manipulation of luminescent bacteria
K. Nealson (1978)
[15] Isolation, identification, and manipulation of luminous bacteriaMethods in Enzymology, 57
C. Balakrishnan, N. Langerman (1977)
The isolation of a bacterial glycoprotein with luciferase activity.Archives of biochemistry and biophysics, 181 2
(1981)
Autoinduction of bacterial bio
M. Nicolas, J. Bassot, Gisele Nicolas (1989)
Immunogold labeling of luciferase in the luminous bacterium Vibrio harveyi after fast-freeze fixation and different freeze-substitution and embedding procedures.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 37
T. Johnston, R. Thompson, T. Baldwin (1986)
Nucleotide sequence of the luxB gene of Vibrio harveyi and the complete amino acid sequence of the beta subunit of bacterial luciferase.The Journal of biological chemistry, 261 11
G. Newman (1987)
Use and abuse of LR White.The Histochemical journal, 19 2
D. Cohn, A. Mileham, M. Simon, K. Nealson, S. Rausch, D. Bonam, T. Baldwin (1986)
Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the alpha subunit of bacterial luciferase.The Journal of biological chemistry, 260 10
G. Mitchell, J. Hastings (1971)
A stable, inexpensive, solid-state photomultiplier photometer.Analytical biochemistry, 39 1
203 152 152 1 1 Pio Colepicolo Marie-Thérèse Nicolas Jean-Marie Bassot J. Woodland Hastings Department of Cellular and Developmental Biology Harvard University 02138 Cambridge MA USA Laboratoire de Bioluminescence CNRS 105 Blvd Raspail 75006 Paris France Abstract Using a polyclonal antibody raised against purified luciferase of Vibrio harveyi and immunogold labeling on thin sections, the amounts and cellular localization of luciferase were examined during the growth of the bacteria. Cells harvested at different times during cultivation in liquid medium at 22°C were fixed, either chemically or by fast freeze fixation followed by freeze substitution, and embedded in LR White. Concomitant measures of bioluminescence, both in vitro and in vivo,showed the classical curve of autoinduction. The number of gold particles per cell area showed a similar pattern. Their localization was always cytoplasmic, with no indication of special periplasmic or membrane associations.
Archives of Microbiology – Springer Journals
Published: Jun 1, 1989
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