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Enhanced cellulase production from Trichoderma reesei QM 9414 on physically treated wheat straw

Enhanced cellulase production from Trichoderma reesei QM 9414 on physically treated wheat straw 110 24 24 3 3 C. Acebal M. P. Castillon P. Estrada I. Mata E. Costa J. Aguado D. Romero F. Jimenez Departamento de Bioquímica Facultad de Química Universidad Complutense 28040 Madrid Spain Departmento de Ingenieria Química, Facultad de Química Universidad Complutense 28040 Madrid Spain Summary Trichoderma reesei QM 9414 was grown on wheat straw as the sole carbon source. The straw was pretreated by physical and chemical methods. The particle size of straw was less than 0.177 mm. Growth of T. reesei QM 9414 was maximal with alkali-pretreated straw whereas cellulase production was optimal when physically pretreated straw was used as substrate. Cellulase yields expressed as IU enzyme activity/g cellulose present in the cultures were considerably higher when alkali pretreatment of wheat straw was omitted. Cellulase yields of 666 IU/g cellulose for filter paper activity (FPA) are the highest described for cultures of T. reesei QM 9414 carried out in analogous conditions. Crystallinity index of the cellulose contained in wheat straw increased slightly after alkali pretreatment. This increase did not decrease cellulose accessibility to the fungus. Delignification of wheat straw was not necessary to achieve the best cellulase production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Microbiology and Biotechnology Springer Journals

Enhanced cellulase production from Trichoderma reesei QM 9414 on physically treated wheat straw

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Publisher
Springer Journals
Copyright
Copyright © 1986 by Springer-Verlag
Subject
Chemistry; Biotechnology; Microbiology; Microbial Genetics and Genomics
ISSN
0175-7598
eISSN
1432-0614
DOI
10.1007/BF00261540
Publisher site
See Article on Publisher Site

Abstract

110 24 24 3 3 C. Acebal M. P. Castillon P. Estrada I. Mata E. Costa J. Aguado D. Romero F. Jimenez Departamento de Bioquímica Facultad de Química Universidad Complutense 28040 Madrid Spain Departmento de Ingenieria Química, Facultad de Química Universidad Complutense 28040 Madrid Spain Summary Trichoderma reesei QM 9414 was grown on wheat straw as the sole carbon source. The straw was pretreated by physical and chemical methods. The particle size of straw was less than 0.177 mm. Growth of T. reesei QM 9414 was maximal with alkali-pretreated straw whereas cellulase production was optimal when physically pretreated straw was used as substrate. Cellulase yields expressed as IU enzyme activity/g cellulose present in the cultures were considerably higher when alkali pretreatment of wheat straw was omitted. Cellulase yields of 666 IU/g cellulose for filter paper activity (FPA) are the highest described for cultures of T. reesei QM 9414 carried out in analogous conditions. Crystallinity index of the cellulose contained in wheat straw increased slightly after alkali pretreatment. This increase did not decrease cellulose accessibility to the fungus. Delignification of wheat straw was not necessary to achieve the best cellulase production.

Journal

Applied Microbiology and Biotechnology – Springer Journals

Published: Jun 1, 1986

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