Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/springer-journals/efficient-production-of-recombinant-protein-in-spodoptera-frugiperda-o0hXVS5Qyh
References (7)
-
H. Johnson (1969)
Purification of b-galactosidaseFungal Genetics Reports, 14
-
David Lindsay, M. Betenbaugh (1992)
Quantification of cell culture factors affecting recombinant protein yields in baculovirus‐infected insect cellsBiotechnology and Bioengineering, 39
-
S. Ha, T. Park, Sam-eun Kim (1996)
Silkworm hemolymph as a substitute for fetal bovine serum in insect cell cultureBiotechnology Techniques, 10
-
S. Reuveny, Y. Kim, C. Kemp, J. Shiloach (1993)
Production of recombinant proteins in high-density insect cell cultures.Biotechnology and bioengineering, 42 2
-
H. Miltenburger (1983)
Investigations on the Cultivation of Insect Cell Lines in Serum-Free Media -
Sung Lee, T. Park (1994)
Growth limiting factors influencing high density culture of insect cells in Grace's mediumBiotechnology Letters, 16
-
(1988)
Bio/technol
- Publisher
- Springer Journals
- Copyright
- Copyright © 1997 by Chapman and Hall
- Subject
- Life Sciences; Biotechnology; Organic Chemistry; Biochemistry, general; Microbiology; Animal Anatomy / Morphology / Histology
- ISSN
- 0141-5492
- eISSN
- 1573-6776
- DOI
- 10.1023/A:1018484309194
- Publisher site
- See Article on Publisher Site
Abstract
Silkworm hemolymph when added at 5% (v/v) to medium increased the production of recombinant β-galactosidase in Spodoptera frugipera/ Autographa californica nuclear polyhedrosis virus (AcNPV) system by 4.5-fold. Silkworm hemolymph also increased the host cell longevity by two-fold. Viability of the host cell was maintained at a constant level for 6 days after baculovirus infection in the medium containing 5% silkworm hemolymph, while host cells began to die 3 days after infection in the medium without hemolymph.
Journal
Biotechnology Letters – Springer Journals
Published: Oct 15, 2004