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Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium `Gower Ramsey'

Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis... The effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis were studied on Oncidium `Gower Ramsey'. Embryo formation was significantly affected by explant position. Leaf tip segments had a significantly higher embryogenic response than other segments of leaves. Adaxial-side-up orientation significantly promoted embryogenesis in comparison with abaxial-side-up orientation. There was no significant effect of sucrose in a range of concentrations (10–60 g l−1). Modified 1/2-MS medium (containing 85 mg l−1 KH2PO4) supplemented with 170 mg l−1 NaH2PO4 significantly promoted direct somatic embryogenesis. Peptone at 0.5 mg l−1 gave significantly higher emrbyogenic response (80%) on leaf tips than control treatment (50%). The best response on direct embryo formation was obtained on the modified 1/2-MS medium supplemented with 10–20 g l−1 sucrose, 170 mg l−1 NaH2PO4 and 0.5 g l−1 peptone. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell, Tissue and Organ Culture Springer Journals

Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium `Gower Ramsey'

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References (13)

Publisher
Springer Journals
Copyright
Copyright © 2002 by Kluwer Academic Publishers
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
0167-6857
eISSN
1573-5044
DOI
10.1023/A:1015004912408
Publisher site
See Article on Publisher Site

Abstract

The effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis were studied on Oncidium `Gower Ramsey'. Embryo formation was significantly affected by explant position. Leaf tip segments had a significantly higher embryogenic response than other segments of leaves. Adaxial-side-up orientation significantly promoted embryogenesis in comparison with abaxial-side-up orientation. There was no significant effect of sucrose in a range of concentrations (10–60 g l−1). Modified 1/2-MS medium (containing 85 mg l−1 KH2PO4) supplemented with 170 mg l−1 NaH2PO4 significantly promoted direct somatic embryogenesis. Peptone at 0.5 mg l−1 gave significantly higher emrbyogenic response (80%) on leaf tips than control treatment (50%). The best response on direct embryo formation was obtained on the modified 1/2-MS medium supplemented with 10–20 g l−1 sucrose, 170 mg l−1 NaH2PO4 and 0.5 g l−1 peptone.

Journal

Plant Cell, Tissue and Organ Culture – Springer Journals

Published: Oct 13, 2004

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